新基因BC047440在多种肿瘤组织中的表达及其意义  

Expression and Significance of A Novel Gene BC047440 in Multiplicate Malignant Tumor Tissues

作　　者：郑璐[1] 李靖[1] 梁平[1] 杨彤翰[1] 黄小兵[1] 刘世呈[1] 

机构地区：[1]第三军医大学新桥医院肝胆外科,重庆400037

出　　处：《中国普外基础与临床杂志》2008年第3期185-189,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(编号:30570843)

摘　　要：目的探讨新基因BC047440在多种恶性肿瘤及对应癌旁组织中的表达,对该基因的癌表达谱进行初步研究。方法采用逆转录聚合酶链反应(RT-PCR)半定量检测肝癌、胆管癌、胃癌、大肠癌、神经胶质瘤、乳腺癌及其相对应的癌旁组织中BC047440 mRNA的表达。结果在48例肿瘤中,BC047440基因的mRNA表达在肝癌、胃癌、胆管癌、大肠癌中较其对应癌旁组织高,差异有统计学意义(P<0.05或0.01);在神经胶质瘤及瘤旁脑组织中均呈高表达,表达量差异无统计学意义(P>0.05);在乳腺癌及其对应癌旁组织中不表达或低表达(P>0.05)。临床病理学分析提示,BC047440基因的表达水平与肝癌及胃肠道肿瘤的生长、侵袭和转移能力有关。结论BC047440基因在肝癌、胃癌、胆管癌、大肠癌及神经胶质瘤中广泛高表达,在这些肿瘤发病过程中可能是一个通过表达升高而发挥功能的结构基因。Objective To study the mRNA expression of BC047440 gene in multiplicate malignant tumor tissues and the corresponding adjacent tissues, and to investigate its roles in the carcinogenesis and development of malignant tumors. Methods Forty-eight cases of malignant tumor tissues and their adjacent non-cancerous tissues were examined. The mRNA expression of BC047440 gene in those tissues of liver cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine, glioma, and breast cancer were measured by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results①The mRNA expressions of BC047440 gene in liver cancer, gastric cancer, cholangiocarcinoma and carcinoma of large intestine were significantly higher than those in their adjacent non-cancerous tissues （P〈0.05 or 0.01）. BC047440 gene were highly expressed in both glioma and its adjacent tissues （P〉0.05）, and poorly expressed in both breast cancer and its adjacent tissues （P〉0.05）. ②There were close relationships between BC047440 gene expression and clinicopathologic findings of liver cancer, including tumor size and portal vein invasion （P〈0.05）. ③There were also close relationships between BC047440 gene expression and different clinical stages in alimentary canal cancers （P〈0. 05）. Conclusion The over expression of BC047440 gene may be related with the growth, infiltration and metastasis of some malignant tilmors, including liv er cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine and glioma.

关 键 词：肿瘤 癌基因BC047440 逆转录聚合酶链反应 半定量 

分 类 号：R730.2[医药卫生—肿瘤]