赛来昔布联合热疗治疗胰腺癌的研究  

The treatment of celebrex combined with thermotherapy for pancreatic carcinoma： in vivo and in vitro

作　　者：刘丽燕[1] 王兴鹏[1] 姜立新[3] 吴恺[1] 杨佳芳[1] 臧丽娟[2] 

机构地区：[1]上海交通大学附属第一人民医院消化科,上海200080 [2]上海交通大学附属第一人民医院病理科,上海200080 [3]上海交通大学附属第六人民医院B超室

出　　处：《中华胰腺病杂志》2008年第1期31-34,共4页Chinese Journal of Pancreatology

摘　　要：目的研究选择性环氧合酶-2抑制剂赛来昔布联合热疗对人胰腺癌生长的抑制作用。方法分别应用热疗、赛来昔布（100μmol／L）及两者联合处理胰腺癌SW1990细胞，MTT法检测细胞增殖，流式细胞仪法检测细胞凋亡。建立裸鼠胰腺癌移植瘤模型，分别给予热疗、赛来昔布及两者联合应用，观察移植瘤的生长、病理改变，RT—PCR检测bcl-2及bax mRNA的表达。结果热疗、赛来昔布及联合组SW1990细胞存活率分别为（56．63±5．78）％、（44．55±4．76）％和（21．83±2．33）％；细胞凋亡比例分别为11．78％、17．33％和33．32％；抑瘤率分别为81．3％、93．0％、99．7％，各组间差异均非常显著（P〈0．01）。以联合组的治疗效果最明显。各组瘤组织bax mRNA表达上调，以联合组上调最明显，各组间差异显著（P〈0．05）；bcl-2表达无变化。结论赛来昔布、热疗在体内外实验中均对胰腺癌治疗有效，两者联合应用具有协同效应。其机制可能是通过上调bax的表达而诱导胰腺癌细胞凋亡。Objective To investigate the anti-proliferative effect of Celebrex （selective cyclooxygenase-2 inhibitor） combined with thermotherapy on pancreatic carcinoma cells. Methods SW1990 pancreatic cancer ceils were treated with thermotherapy or Celebrex or the combination. MTT was used to evaluate the cell viability. Flow cytometry was used to observe the cell apoptosis. Pancreatic cancer xenograft tumor model was established in nude mice, and then divided into 3 groups： thermotherapy group, Celebrex group and combination group. RT-PCR was used to detect the expression of bcl-2 mRNA, bax mRNA both in vivo and in vitro. Result All treated groups had an obviously inhibitory effect on the growth of SW1990 cells, and the cell survival rate were 56.63 ±5.78%, 44.55 ± 4.76 % and 21.83 ± 2.33%, respectively. Apoptosis ratios were 11.78%, 17.33% and 33.32%, respectively. Tumor inhibitory rates were 81.3%, 93.0%, 99.7% , respectively. The difference between these groups were significant （P 〈 0.01 ）. RT-PCR showed bax was up-regulated in all groups, especially in the combination group （P 〈 0.05 ）. However bcl-2 expression was not different between these groups. Conclusions Celebrex combined with thermotherapy had a synergistic effect in the treatment of pancreatic carcinoma both in vivo and in vitro, and the possible mechanism may be up-regulating bax expression to induce tumor cell apoptosis.

关 键 词：胰腺肿瘤 环氧化酶2抑制剂 高温 诱发 bel-2相关x蛋白质 

分 类 号：R735.9[医药卫生—肿瘤]