银杏黄酮甙对糖尿病大鼠肾组织PAI-1、MMPs-3表达的影响  

Effects of Ginkgo Flavonal on the expression of PAI-1、MMPs-3 in diabetic rats

作　　者：侯淑芬[1] 刘秋菊[1] 张立新[2] 张春雪[1] 赵芬宣[1] 晋素景[1] 

机构地区：[1]河北大学附属医院肾内科,河北保定071000 [2]河北大学医学部电教中心,河北保定071000

出　　处：《河北职工医学院学报》2008年第1期10-11,20,共3页Journal of Hebei Medical College for Continuing Education

基　　金：河北省保定市科技攻关计划项目(编号:04F002)

摘　　要：目的探讨银杏黄酮甙对糖尿病大鼠肾脏保护作用机制。方法选择雄性SD大鼠48只,随机分为3组,每组16只,即正常对照组(A组),糖尿病组(B组),银杏黄酮甙治疗组(C组)。B、C组腹腔注射链脲佐菌素(STZ)制模成功后,C组给予银杏黄酮甙灌胃,10周后将大鼠宰杀,用免疫组化方法观察肾脏PAI-1、MMPs-3的表达。结果与A组相比免疫组化:PAI-1第6周时B、C组均高于A组(P<0.01,P<0.05),持续至第10周(P<0.01),但C组低于B组(P<0.01)。MMPs-3第6周时B组低于A组(P<0.05),持续至第10周(P<0.01),C组则高于A、B组(P<0.01)。结论STZ诱导的糖尿病大鼠PAI-1表达增强、MMPs-3表达下调。应用银杏黄酮甙治疗,可以使PAI-1的表达下调,MMPs-3的表达上调,对糖尿病大鼠肾脏有保护作用。Objective To investigate the role of Ginkgo Flavonol on the mechanism of protecting kidney in diabetic rats. Methods：Male Sprague- Dawley rats weighting 180- 200g were randomly divided into three groups： the control rats （Group A）, the diabetic rats（Group B）and the diabetics rats treated with Ginkgo Flavonol （Group C）. Streptozotocin （STZ） 65mg/kg body weight, were celioinjected in Group B and Group C. Group C was treated with Ginkgo flavonol. 6 weeks and 10 weeks after the treatment with Ginkgo flavolon , the following studies were performed in every group. The renal tissue dealt with formalin were obtained, Immunohistochemical technique （SP assay） was used to estimate the expression of matrix metalloproteinases - 3 （ MMPs - 3 ）, Plasminogen activator inhibitor - 1 （ PAI - 1 ） in renal cortex and the renal tissue of diabetic rats were observed. Results： The level of renal PAI- 1 were higher in Group B, C than in Group A（ P 〈 0. 01, P 〈 0. 05）, In 10 weeks, its level in Group C lower than that of in Group B（ P 〈 0. 05 ） ; The level of renal MMPs - 3 were lower in Group B than in Group A（ P 〈 0. 05）, But its lovel in Group C higher than in Group A, B（ P 〈 0. 01 ）. Conclusion The results indicate that STZ - induced diabetic rats exist the strong expression of renal PAI - 1, the low expression on renal MMPs - 3, Ginkgo Flavonol down - regulate the expression of PAI- 1, and up - regulate the expression of MMps - 3. Ginkgo Flavonol play a role in protecting diabetic rats from nephropathies.

关 键 词：糖尿病肾病 银杏黄酮甙 血浆纤溶酶原激活物抑制物(PhI-1) 基质金属蛋白酶-3(MMPs-3) 

分 类 号：R-332[医药卫生]