骨髓间充质干细胞上清液治疗大鼠脑缺血的作用机制  

The Machenism of Supernatant from Bone Marrow Stromal Cells in Rats for the Treatment Cerebral Ischemia

作　　者：冯鹏[1] 李光来[2] 王飞[1] 

机构地区：[1]山西医科大学 [2]山西医科大学第二医院

出　　处：《中西医结合心脑血管病杂志》2008年第3期292-294,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的观察骨髓间充质干细胞上清液经尾静脉注射治疗大鼠脑缺血的干预效果,探讨骨髓间充质干细胞移植体内作用机制。方法建立大鼠大脑中动脉缺血再灌注模型。16只健康Wistar大鼠分为假手术组、缺血对照组、上清液低浓度组、上清液高浓度组。模型制作24h后各组腹腔注射5-溴脱氧尿核苷(bromodeoxyuridine,BrdU)标记处于增殖状态的神经前体细胞,应用免疫组织化学染色检测缺血后7d脑组织BrdU、BrdU+NSE、BrdU+GFAP阳性细胞数。结果脑梗死后7d侧脑室室管膜下区、海马齿状回区BrdU、BrdU+NSE、BrdU+GFAP阳性细胞数开始增多,上清液高浓度组的上述阳性细胞数明显多于对照组(P<0.01)和低浓度组(P<0.05)。结论骨髓间充质干细胞上清液可以促进脑梗死大鼠损伤原位内源性神经前体细胞的增殖、分化。Objective To observe the effect of supematant from bone marrow stromal cells（BMSCs）on the proliferation and differentiation of endogenous neural precursor cells in rats after cerebral ischemia and reperfusion. Methods Sixteen Wistar rat models with middle cerebral artery occlusion （MCAO） and reperfusion were divided into four groups：Sham- operated group, ischemia control group, low concentration supematant group and high concentration supematant group. BrdU that labeled the proliferating neural precursor cells were injected intraperitoneally in models after cerebral ischemia and reperfusion for 24 hours. At the end, the positive cells labeled by BrdU, BrdU ＋ NSE （Neuron specific enolase） and BrdU ＋ GFAP（Glial fibrillary acidic protein）were counted by immunohistochemistrical method at the 7th day after ischernia and reperfusion. Results The number of positive cells labeled by BrdU, BrdU ＋ NSE and BrdU ＋ GFAP in the ependyma/subventricular zone and hippocampus increased at the 7th day after ischemia and reperfusion. At ,same time, compared with the isehemia control group and low concentration supernatant group, the number of three kinds of positive cells in high - concentration supernatant group increased significantly in above zones （P 〈 0.05）. Conclusion The supernatant from BMSCs oauld promote the proliferation and differentiation of endogenous neural precursor cells after cerebral ischomia.

关 键 词：脑缺血 骨髓间充质干细胞 上清液 神经前钵细胞 增殖 分化 

分 类 号：R743[医药卫生—神经病学与精神病学]