iNOS和HIF1α在骨肉瘤中的表达及其临床病理相关性研究  

Study on expressions of HIF1 αand iNOS in osteosarcoma and their correlations with clinical and pathological features

作　　者：耿艳华[1] 王昌兴[2] 陈培辉[1] 

机构地区：[1]浙江大学医学院附属二院,310009 [2]浙江省新华医院,310005

出　　处：《浙江临床医学》2008年第3期291-292,共2页Zhejiang Clinical Medical Journal

摘　　要：目的从酶蛋白水平研究骨肉瘤组织缺氧代谢过程中iNOS、HIF1α的表达及与临床、病理特征的关系;探讨它们在肿瘤发展过程中作用的可能机制。方法筛选不含硬骨组织,未经脱钙处理的骨肉瘤标本59例,依据临床病理特征进行分组,并行术后随访。应用免疫组织化学EnVision法检测肿瘤组织中iNOS、HIF1α表达,应用χ2检验、Spearman等级相关分析等进行统计分析。结果骨肉瘤中iN-OS、HIF1α表达阳性率分别为86·44%、38·98%,对照组中分别为9·9%和0,两组比较,差异均有显著性。HIF1α的表达与骨肉瘤患者年龄有关(P<0·01),iNOS则无关(P>0·05)。HIF1α和iNOS的表达均与骨肉瘤组织学类型有关,iNOS、HIF1α在髓内高分化型和骨旁型骨肉瘤总阳性表达率分别为33·33%和0,分别低于其余各组总和阳性率91·30%和41·30%,差异有显著性(P<0·01和0·05)。HIF1α与iNOS表达正相关,两者共阳率38·98%,共阴率13·56%。iNOS阳性与阴性组中,HIF1α的阳性表达率分别为45·10%和0,差异有显著性(P<0·01)。结论iNOS与HIF1α在骨肉瘤组织中均高表达,与肿瘤的发生、发展有关,可能协同促进肿瘤的恶性进展。Objective To investigate the expressions of HIF1α and iNOS in 59 cases of osteosarcoma and their correlations with clinical and pathological featm＇es, and to explore their roles in tumor development . Methods 59 cases typical osteosarcoma specimens without os ossenm tissue and non - decalcification were obtained and grouped according to the clinicopathologic characteristics, the followed- up data of postoperative patients were collected. ＇Fne tests were examined by immunohistechemical staining （EnVision）. Data were evaluated by X^2 and Spearman rank correlation analysis, the survival data of postoperative were performed by kaplan - meier survival curve analysis and Log- Rank method. Results ＇Fne positive expression rates of iNOS and HIF1α in osteosarcoma were 86.44 % and 38.98 % respectively , which were significantly higher than that of control group （ P 〈 0.01 and 0.05 ）, respectively. ＇Fne expression of HIF1α was positively related with the age （ P 〈 0.01 ）, but the iNOS was not （ P 〉 0.05 ）. Tne expression of HIF1α and iNOS were positively related with the histological classifications, in iNOS and HIF1α positive groups, total positive rates of intraosseous well - differentiated and parostea osteosarcoma was 33.33% and 0, respectively, which was remarkably lower than that of the other groups （91.30% and 41.30%, ） with significant difference （ P 〈 0.01 and 0.05, respectively）. Among the samples , HIF- 1αand iNOS both expressed in 38.98%, no expression of either of them in 13.56%, the positive expression rate of HIF - 1α in the group of positive and negative iNOS were 45.10% and 0, respectively, which showed a compacted positive correlation between them （ P 〈 0.01 ）. Expressions of HIF1αdemonstmted negative correlation with the postoperative survival time（ P 〈 0.01 ）, but the iNOS didn＇t （ P 〉 0.05）. Conclusions iNOS and HIF1α are expressed in osteosarcoma tissue . ＇Fney play a cooperative role in influencing the growth of tumor. The expressi

关 键 词：骨肉瘤 诱导型-氧化氮合酶 缺氧诱导因子1Α 免疫组织化学 

分 类 号：R738.1[医药卫生—肿瘤]