EBV相关胃癌组织中ras基因突变的检测  

A STUDY OF ras GENE MUTATION IN EPSTEIN-BARR VIRUS-ASSOCIATED GASTRIC CARCINOMA

作　　者：孙佰秀[1] 刘薇[1] 张璐[1] 罗兵[2] 

机构地区：[1]青岛市妇女儿童医疗保健中心检验科,山东青岛266011 [2]青岛大学医学院微生物学教研室

出　　处：《青岛大学医学院学报》2008年第1期21-23,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨EBV感染、ras基因突变在EBV相关胃癌(EBVaGC)发生发展中的作用。方法应用PCR-RFLP技术,分别检测13例EBVaGCs、45例与之匹配的EBV阴性胃癌(EBVnGC)以及58例相应癌旁组织中H-ras 12位点,K-ras 12、13位点突变情况。结果检测到2例H-ras 12位点突变,突变率为4.44%(2/58),均发生在EBVnGC组织。结论胃癌组织ras基因突变率较低,ras基因突变与EBVaGC的发生无明显相关性。Objective To analyze the pathogenic role of EBV infection and ras mutations in the development of EBV-associated gastric carcinoma （EBVaGC）. Methods Ras gene mutations were tested by PCR-RFLP assay in 13 EBVaGCs, 45 EBV- negative gastric carcinomas （EBVnGC） with matched clinicopathological parameters and 58 corresponding adjacent tissues of the cancer. Results H-ras 12 codon mutation was detected in two cases of 58 gastric carcinomas （4.44%）, which were in EBVnGC. Conclusion The rate of ras gene mutations in gastric carcinomas is low. Ras gene mutation is not related with the development of EBVaGC.

关 键 词：胃肿瘤 疱疹病毒4型 人 基因 ras 

分 类 号：R735.2[医药卫生—肿瘤]