N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血清诱导的心脏成纤维细胞MMP-2、MMP-9活性表达的调节作用  

Effect of AcSDKP on activities of MMP-2 and MMP-9 in cardiac fibroblast induced by FBS

作　　者：马文东[1] 张丽娟[1] 罗玲[1] 裴新[1] 户万秘[1] 杨方[1] 

机构地区：[1]华北煤炭医学院实验中心,河北唐山063000

出　　处：《华北煤炭医学院学报》2008年第2期147-148,共2页Journal of North China Coal Medical College

基　　金：河北省科技厅博士基金(04547002D-5)资助项目;河北省教育厅基金(2003112)资助项目;唐山市新医药基础研究重点实验室项目(04362001B)

摘　　要：①目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对不同浓度血清(FBS)诱导的大鼠心脏成纤维细胞MMP-2、MMP-9酶活性表达的调节作用。②方法选用新生大鼠心脏成纤维细胞;采用明胶酶谱法检测心脏成纤维细胞MMP-2、MMP-9酶活性的表达。③结果在0.4%、2%和10%几种血清浓度的条件下,随着血清浓度的增加,MMP-2、MMP-9酶活性表达增强。AcS-DKP能够进一步增加由10%血清诱导的心脏成纤维细胞MMP-2、MMP-9酶活性的表达。④结论AcSDKP上调了由血清介导的心成纤维细胞MMP-2,MMP-9酶活性表达,这可能与AcSDKP抗心脏纤维化的作用相关。Objective To investigate the regulation effects of AcSDKP on activity expressions of MMP - 2 and MMP - 9 in cultured rat cardiac fibroblasts mediated by different concentration fetal bovine senrum（FBS） . Methods Neonatal rat cardiac fibroblasts were isolated. The MMP -2 and MMP -9 activity expressions were measured with zymography agsays. Results MMP -2 and MMP -9 activity expressions were increased as FBS increase at the 0.4% , 2% and 10% concentrations. MMP -2 and MMP -9 activity expression in cardiac fibroblasts induced by 10% FBS were further increased by AcSDKP. Conclusion MMP -2 and MMP -9 activity expressions in cardiac fibroblasts mediated by FBS were up -regulated by AcSDKP ,which was possible related with the effect of AcSDKP anti - fibrosis.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血清 心脏成纤维细胞 基质金属蛋白酶 

分 类 号：R362[医药卫生—病理学]