唐古特大黄多糖促进IEC-6细胞增殖、移行作用及其可能的机制研究  

Promoting effects of Rheum tanguticum polysaccharide on IEC-6 cell proliferation,migration and its possible mechanism

作　　者：刘琳娜[1] 梅其炳[2] 王志鹏[2] 张琰[1] 刘莉[2] 刘新友[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038 [2]第四军医大学药理学教研室,陕西西安710032

出　　处：《中国药理学通报》2008年第3期303-307,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30100239)

摘　　要：目的观察唐古特大黄多糖组分之一(RTP1)对大鼠小肠隐窝上皮细胞株(IEC-6)细胞增殖、移行及其对细胞内鸟氨酸脱羧酶(ODC)活性和ODC蛋白表达的影响,观察其对胃肠黏膜的修复作用并探讨可能的机制。方法采用MTT法和体外肠上皮细胞移行模型,观察不同剂量的RTP1(10、30、100mg·L-1)处理IEC-6细胞24h后,细胞增殖和移行情况;Western blot检测ODC蛋白表达;通过测定L-[1-14C]鸟氨酸释放14CO2的量来检测ODC活性变化。结果RTP1在10、30、100mg·L-1时均可明显促进IEC-6细胞的增殖和移行,可增加ODC蛋白表达和ODC活性,并具有剂量依赖性,与空白对照组相比差异具有显著性(P<0·05或0·01)。结论RTP1可促进肠上皮细胞的增殖和移行,提示RTP1可能对肠黏膜的损伤修复具有直接作用。其作用机制可能与鸟氨酸脱羧酶表达增加和(或)活性增大有关。Aim To observe the effects of Rheum tanguticum Polysaccharide （ RTP1 ） on promoting intestinal epithelial cell line 6 （IEC-6） proliferation and mi gration , and to detect whether, these effects were induced by activating the activity of ornithine decarboxylase （ODC） and promoting ODC protein expression. Thus the gastrointestinal mucosa healing effect of RTP1 could be detected, as well as its possible mechanism. Methods After the cells were pretreated with RTP1 for 24 h （10, 30,100 mg · L^-1） , MTT assay and in vitro cellular wound restoration model were introduced to observe the effects of RTP1 on cell proliferation and restitution. Western blot analysis was used to detect ODC protein expression. ODC activity was assessed by measuring the amount of 14CO2 liberated from DL-[ 1 - ^14C] ornithine. Results RTP1 dose-dependently enhanced IEC-6 cell proliferation and restitution. ODC protein expression and ODC activity were also increased as a result of this process. As compared with the control, the difference was significant （ P 〈 0. 05 or 0. 01 ）. Conclusions Pretreated with RTP1 could promote IEC-6 cell proliferation and restitution. These results suggested that RTP1 might have a direct mucosal healing effect on intestinal epithelial cells. And the possible mechanism might be related with activating the activity of ODC and/or promoting ODC protein expression.

关 键 词：唐古特大黄多糖 IEC-6细胞 增殖 移行 鸟氨酸脱羧酶 

分 类 号：R-332[医药卫生] R284.1