造模持续时间对大鼠高营养性肥胖模型制备的影响  

Effect of duration of preparation on the formation of DIO model in rats

作　　者：崔明霞[1] 高琲[1] 闵光宁[1] 王莉[1] 路莉[1] 张宝来[1] 覃红[1] 高明堂[1] 李文广[1] 郑荣梁[2] 吴勇杰[1] 

机构地区：[1]药学院药理学研究所,甘肃省新药临床前研究重点实验室,甘肃兰州730000 [2]兰州大学生命科学学院,甘肃兰州730000

出　　处：《中国药理学通报》2008年第3期412-416,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30600770);甘肃省自然科学基金资助项目(No3ZS051-A25-091)

摘　　要：目的研究造模持续时间对大鼠高营养饮食诱导型肥胖(DIO)及其相关指标形成的影响。方法在3批实验中,断乳♂SD大鼠各290只,用相同配方的高营养饲料分别喂养7、9和10wk以建立DIO模型,然后分别给西布曲明8、4和4mg·kg-1共18、14和14d。实验指标包括体重、Lee′s指数、肝脏和腹腔脂肪湿重、血糖和血脂水平以及脂肪肝的形成。结果造模7wk的DIO大鼠体重、Lee′s指数、肝脏和脂肪湿重高于正常组;造模9wk的除以上指标有改变外,血TG水平也升高;造模10wk后,又同时出现血GLU水平的升高,且有明显的脂肪肝形成。结论造模的持续时间对DIO大鼠肥胖相关指标的形成有影响,应根据实际研究目的确定模型建立所需时间的长短。Aim To study the effect of duration of preparation on high-nutrition diet induced obesity （DIO） model and obesity related indices in rats. male SD rats were fed with high-nutrition diet for 7, 9 and 10 weeks and administrated sibutramine 8, 4 and 4 mg· kg^-1 for 18, 14 and 14 days in experiment 1, 2 and 3, respectively. The indices of experiments were body weight, Lee＇s index, liver and visceral fat wet weight, blood lipid and glucose level and the formation of fatty liver. Results The body weight, Lee＇s index, liver and visceral fat wet weight of DIO rats were significantly higher than that of normal rats when duration of model preparation was 7 wk. The hypertriglyceridemia appeared at week 9. At week 10, hyperglycemia and fatty liver appeared in addition to the significant changes of indices above. Conclusion The duration of model preparation had obvious effects on the formation of obesity related indices in DIO rats and should be determined according to the objective of the study.

关 键 词：肥胖 大鼠 模型 西布曲明 

分 类 号：R-332[医药卫生] R329.24