野生芋属植物干叶片DNA的提取及PCR扩增  

DNA Extraction from Dried Leaves and PCR Amplification of Colocasia

作　　者：蔡秀珍[1] 刘克明[1] 龙春林[2] 

机构地区：[1]湖南师范大学生命科学学院植物学系,湖南长沙410081 [2]中国科学院昆明植物研究所,云南昆明650204

出　　处：《中国野生植物资源》2008年第1期51-53,57,共4页Chinese Wild Plant Resources

基　　金：国家自然科学基金(30170102和30470130);国家科技基础条件平台项目(2005DKA210006)

摘　　要：野生芋属植物体内多糖、色素、酚类等次生物质含量较高,严重影响从中提取的DNA的质量。针对这一问题,作者以6种芋属植物的干叶片为材料,摸索出一种适合芋属植物的DNA提取方法,并对提取的DNA进行了纯度鉴定和PCR检测,结果表明此方法可有效去除次生物质对DNA的干扰,样品DNA的质量和纯度较高,可用于下游分子生物学操作。The contents of polysaccharides, pigment, phenolic compound and other secondary metabolites are high in Colocasia Schott, which influence frequently the yield and quality of DNA when being extracted. In this study, a new method was introduced which do best for characterization the purity and concen- tration of DNA. The result showed that it could effectively eliminate the affections of the secondary materials to extracted DNA from Colocasia, and which be suitable for other molecular analysis.

关 键 词：DNA提取 多糖 色素 酚类 

分 类 号：Q523[生物学—生物化学]