PPARγ配体Ciglitazone对人胃癌MGC803细胞增殖的影响及其机制  

Effect of PPARγ Ligand Ciglitazone on Human Gastric Carcinoma MGC803 Cells Proliferation and Its Mechanism

作　　者：马秀梅[1] 任明姬[2] 

机构地区：[1]内蒙古医学院病理教研室,呼和浩特010059 [2]内蒙古医学院组织胚胎教研室,呼和浩特010059

出　　处：《肿瘤防治研究》2008年第3期164-168,共5页Cancer Research on Prevention and Treatment

摘　　要：目的探讨PPARγ配体Ciglitazone对人胃癌MGC803细胞增殖的影响及其机制。方法RT-PCR检测MGC803细胞PPARγmRNA。MTT及流式细胞术检测Ciglitazone对MGC803细胞增殖及凋亡的影响。相差倒置显微镜和Hoechst33342染色观察凋亡的形态学变化。免疫组化和流式细胞术检测PPARγ、Bcl-2、Bag-1及Bax蛋白表达和变化。结果PPARγ配体Ciglitazone(5、10、20和50μmol/L)可抑制MGC803细胞增殖和诱导其凋亡,增殖的抑制和凋亡的诱导有平行性,且有浓度和时间依赖性。随20μmol/L Ciglitazone作用时间的延长,MGC803细胞的PPARγmRNA及蛋白表达、Bax蛋白表达及Bax/Bcl-2蛋白的比值升高,Bcl-2和Bag-1蛋白表达降低。结论Ciglitazone可能主要通过激活PPARγ抑制人胃癌MGC803细胞增殖和诱导其凋亡。Bax蛋白表达和Bax/Bcl-2蛋白表达比值升高,Bcl-2和Bag-1蛋白表达降低在Ciglitazone诱导人胃癌MGC803细胞凋亡中起重要作用。提示Ciglitazone可能对治疗胃癌有效。Objective To investigate effect of peroxisome proliferator-activated receptorγ（PPARγ）ligand Ciglitazone on proliferation of human gastric carcinoma MGC803 cells and its mechanism. Methods To examine PPARγmRNA of MGC803 cells by RT-PCR. To examine effect of PPARγ ligand Ciglitazone on MGC803 cells proliferation and apoptosis by MTT and Flow Cytometry. To examine morphological change of apoptosis by phase contrast microscope and Hoechst33342 staining. To examine the expression and change of PPARγ, Bcl-2, Bag-1 and Bax protein of MGC803 cells by immunohistochemistry and Flow Cytometry. Results Ciglitazone（5 μmol/L, It） μmol/L,20 μmol/L and 50 μmol/L） inhibited proliferation and induced apoptosis of MGC803 cells and apoptosis is parallel to proliferation inhibition in MGC803 cells, which were dose- and time-dependent. PPARγmRNA and protein expression, Bax protein expression and Bax protein over Bcl-2 protein ratio were enhanced and both Bcl-2 and Bag-1 protein expression were decreased with prolonged time following 20μmol/L Ciglitazone treatment in MGC803 cells. Conclusion Ciglitazone may inhibit proliferation and induce apoptosis in human gastric carcinoma MG803 cells via activating PPARγ. Elevated Bax protein expression and Bax protein over Bcl-2 protein ratio and decreased Bcl-2 and Bag-1 protein expression may play an important role during apoptosis of human gastric carcinoma MGC803 cells induced by Ciglitazone, which suggest that Ciglitazone may effective to treat gastric carcinoma.

关 键 词：PPARΓ CIGLITAZONE 胃癌 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]