费约果外植体灭菌及愈伤组织的诱导  

Sterilization of the Explant and Callus Induction of Feijoa sallowiana Berg.in vitro Culture

作　　者：周丽娟[1] 王丹[1] 黄海涛[1] 袁涛[1] 张晓雪[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《热带亚热带植物学报》2008年第2期179-183,共5页Journal of Tropical and Subtropical Botany

基　　金：四川省科技厅国际科技合作项目(2007H12-004)资助

摘　　要：以费约果(FeijoasallowianaBerg.)嫩叶和带芽茎段作为外植体,研究费约果愈伤组织诱导的最佳条件和方法。结果表明:(1)最佳基本培养基是MS;(2)二步灭菌法优于一步灭菌法;(3)嫩叶和带芽茎段愈伤组织诱导的最佳活性炭浓度分别为2.0gL-1和3.0gL-1;(4)最佳青霉素浓度分别为200mgL-1和50mgL-1;(5)光培养方式优于暗培养方式。(6)费约果嫩叶诱导愈伤组织的最佳培养基是MS+2.0mgL-12,4-D+1.0mgL-1KT(或0.1mgL-1NAA);(7)带芽茎段的最佳培养基是MS+1.0mgL-16-BA+0.5mgL-1NAA+1.0mgL-1GA或MS+2.0mgL-16-BA+0.1mgL-1NAA+1.0mgL-1GA。The optimum conditions for the tissue culture of Feijoa （Feijoa sallowiana Berg.） were investigated. The leaves and shoot with bud were used as explants. The results showed that the optimal basal medium was MS medium. Two-step sterilization was better than One-step sterilization. The optimal concentration of active carbon for callus induction from leaves and shoot with bud were 2.0 g L^-1 and 3.0 g L^-1, respectively, and penicillin were 200 mg L^-1 and 50 mg L^-1, respectively. The culture under light was better than under dark. The optimal medium for callus induction from tender leaves was MS ＋ 2.0 mg L^-1 2,4-D ＋ 1.0 mg L^-1 KT （or 0.1 mg L^-1 NAA）, and from shoot with bud was MS ＋1.0 mg L^-1 6-BA＋0.5mg L^-1 NAA＋ 1.0 mg L^-1 GA, or MS ＋2.0 mg L^-1 6-BA＋ 0.1 mg L^-1 NAA ＋ 1.0 mg L^-1 GA.

关 键 词：费约果 组织培养 嫩叶 带芽茎段 

分 类 号：Q943.1[生物学—植物学]