副结核分枝杆菌SOD基因的克隆与序列分析  

Cloning and Sequence Analysis of Mycobacterium paratuberculosis SOD Gene

作　　者：曾凡利[1] 胡玉庆[1] 徐凤宇[1] 王春芳[1] 姜秀云[2] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]吉林农业大学生命科学学院,长春130118

出　　处：《中国兽药杂志》2008年第3期1-3,共3页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金(30471285)

摘　　要：以副结核分枝杆菌C-2染色体DNA为模板,以SOD基因特异性引物进行PCR扩增,获得约620 bp的DNA片段。将PCR产物克隆至pGEM-T Vector中,通过α-互补法筛选和质粒酶切及序列分析鉴定,成功构建出重组质粒pGEM-T-SOD,为进一步研究SOD基因及其表达产物的免疫生化特性奠定基础。The genomic DNA was extracted from Mycobacterium paratuberculosis C2,the mature secreted protein SOD gene was amplified with a pair of specific primers through using polymerase chain reaction（PCR）.The PCR product was approximately 620 bp DNA segment.The PCR product was cloned into pGEM-T vector,then by using α-complementation test,restrictional enzyme assay and recombinant plasmid sequence analysis,Recombinant plasmid pGEM-T-SOD was successfully constructed.These results could serve as a basis for further studies on the usefulness of SOD gene and immunogenicity of SOD gene expression product.

关 键 词：副结核分枝杆菌 SOD基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]