二肽基肽酶II在正常大鼠眼球各组织中的免疫表达  

Immune expression of dipeptidyl peptidase II in the normal rat eyeball tissues

作　　者：张辉[1] 秦秀虹[1] 崔永日 杜淑芳[1] 安海鸥[1] 

机构地区：[1]吉林大学第二医院眼科医院,中国吉林省长春市130041 [2]中国吉林省梅河口市医院爱民医院眼科,135000

出　　处：《国际眼科杂志》2008年第3期491-493,共3页International Eye Science

基　　金：中国教育部归国留学人员科研启动基金资助课题(No.2005);中国吉林省长春市科技发展计划资助课题(No.03-1188527);中国吉林省科技发展计划项目(No.200705233)~~

摘　　要：目的:探讨二肽基肽酶II(dipeptidyl peptidaseII,DPPII)在正常大鼠眼球各组织中的免疫表达。方法:应用抗生物素蛋白-生物素-过氧化物酶复合体法,检测DPPII在正常大鼠眼球各组织中的免疫表达。结果:发现DPPII在角膜上皮细胞、角膜基质层、角膜内皮细胞、睫状体无色素上皮细胞、虹膜色素上皮细胞、晶状体上皮细胞、晶状体纤维、视网膜神经纤维层、神经节细胞、视网膜色素上皮细胞中免疫染色均呈阳性。结论:DPPII存在于角膜上皮细胞、角膜基质层、角膜内皮细胞、睫状体无色素上皮细胞、虹膜色素上皮细胞、晶状体上皮细胞、晶状体纤维、视网膜神经纤维层、神经节细胞、视网膜色素上皮细胞。AIM - To investigate the immune expression of dipeptidyl peptidase Ⅱ （DPPⅡ） in the normal eyeball tissues. METHODS： The eyes were removed after the rats were sacrificed and postfixed with 40g/L formalin for 20 hours, dehydrated in ethanol, substituted with bezene, and embedded in paraffin wax. Paraffin sections about 4μm thick were cut by using a microtome and mounted on slides. Then the immune expression of DPPⅡ in the normal eyeball tissues were investigated with ABC method. RESULTS： DPPⅡ found in corneal epithelial cells, corneal stroma, corneal endothelial cells, ciliary non-pigment epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fiber, retinal nerve fiber layer, ganglion cells, retinal pigment epithelium cells showed positive staining. CONCLUSION： DPPⅡ are primarily in the corneal epithelium cells, corneal stroma layer, corneal endothelial cells, ciliary non-pigment epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fiber, retinal nerve fiber layer, ganglion cells, retinal pigment epithelium cells.

关 键 词：二肽基肽酶Ⅱ WISTAR大鼠 眼球 免疫组织化 学法 

分 类 号：R77[医药卫生—眼科]