补肾益气化瘀解毒法对肝纤维化大鼠肝细胞凋亡及凋亡相关基因的影响  

Effects of the Therapy of Invigorating the Kidney and Qi and Dissipating Blood Stasis to Removing the Toxin on the Hepatocyte Apoptosis and Associated Genes in the Liver Fibrosis Rats

作　　者：严茂祥[1] 陈芝芸[1] 宋建华[1] 项柏康[1] 曲宝戈[1] 

机构地区：[1]浙江省中医院消化病研究室,杭州310006

出　　处：《医学研究杂志》2008年第3期29-32,共4页Journal of Medical Research

基　　金：浙江省中医药科研计划(2000C26)

摘　　要：目的观察补肾益气化瘀解毒作用的中药复方肝力克对肝纤维化大鼠肝细胞的凋亡及其相关基因的影响,探讨中药治疗肝纤维化的作用机制。方法以四氯化碳诱导建立大鼠肝纤维化模型,以不同剂量的中药肝力克进行治疗;光镜观察肝组织的病理学变化,采用TUNEL法检测肝细胞的凋亡情况,免疫组化法检测凋亡相关蛋白的表达。结果中药肝力克能显著改变肝纤维化的病理状态,能减轻肝细胞的凋亡情况,并能调节凋亡相关基因的表达。结论肝力克抗纤维化作用的机制可能是通过调节肝纤维化大鼠肝细胞的凋亡水平;抑制肝纤维化大鼠肝细胞Fas/FasL、Bcl-2/Bax的表达来实现的。Objective To investigate the effects of the therapy of invigorating the kidney and qi and dissipating blood stasis to removing the toxin on the hepatocyte apoptosis and associated genes in the liver fibrosis rats, and to explore the treatment mechanism of liver fibrosis with TCM. Methods liver fibrosis model rats were induced by CCl4,and the Chinese herbs （Ganlike） with invigorating the kidney and qi and dissipating blood stasis to removing the toxin was given by daily garage, after the experiment, the pathological changes of liver were observed with optical microscope. Hepatocyte apoptosis was detected with TUNEL assay, apoptosis -associated genes were detected with immunohistochemistry assay. Results The Chinese herbs Ganlike could obvously improve the pathological changes, alleviate the hepatocyte apoptosis and modulate the expression of apoptosis - associated genes. Conclusions The anti - fibrosis mechanism of Ganlike may be to regulate the hepatocyte apoptosis of liver fibrosis rats and to restrain the expressions of hepatocyte Fas/FasL,Bcl- 2/Bax.

关 键 词：肝纤维化 中医药疗法 凋亡 基因 补肾益气化瘀解毒 

分 类 号：R735.2[医药卫生—肿瘤] R575.2[医药卫生—临床医学]