Ubiquitin B在宫颈癌细胞凋亡中的作用及机制的初步探讨  

The possible role of ubiquitin B in HeLa cells' apoptosis and its rudiment mechanism

作　　者：陈彩虹[1] 韩志强[1] 洪振亚[1] 孙立石[1] 卢运萍[1] 周剑锋[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉430030

出　　处：《现代妇产科进展》2008年第1期19-22,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:30371657)

摘　　要：目的:研究Ubiquitin B的过度表达对宫颈癌细胞凋亡的影响,并初步探讨其机制。方法:脂质体转染Ubiquitin B至宫颈癌细胞系HeLa细胞后,real-time PCR检测细胞内Ubiquitin B mRNA的表达水平;流式细胞术和DNA Ladder法检测其凋亡;PI法检测细胞周期变化;Western blot法检测凋亡相关蛋白Smad4和Mcl-1的表达。结果:转染Ubiquitin B后,HeLa细胞中Ubiquitin B mRNA的表达水平在转染24h后明显高于它在对照组细胞中的表达。与对照组相比,转染了Ubiquitin B的HeLa细胞凋亡显著增加。但Ubiquitin B的过表达对HeLa细胞的周期无明显影响。Western blot分析表明,凋亡相关蛋白Smad4在转染Ubiquitin B48h时表达增强,而Mcl-1表达明显减弱。结论:UbiquitinB可促进宫颈癌细胞系HeLa细胞凋亡,其机制可能是Ubiquitin B通过增强表达凋亡促进蛋白Smad4和降解凋亡抑制蛋白Mcl-1而促进了HeLa细胞的凋亡。Objective：To investigate the effect of overexpression of ubiquitin B on HeLa cells＇apoptosis and its rudiment mechanism. Methods：pcDNA3.1 ＋/ubiquitin B was trans-fected into HeLa cells mediated by Lipofectamine 2000. The expression of ubiquitin B mRNA was defected by real-time PCR;the apoptotic rate of HeLa cells was detected by flow cytometry and DNA ladder. PI detected the change of cell circle ; the related protein of ubiquitin B Smad 4 and Mcl-1 were detected by Western-blot assay. Results：Mter transfection, the expression of ubiquitin B mRNA was much higher in the group transfected with ubiquitin B after 24 hours ; the apoptotic rate of HeLa cells was promoted compared with other control groups, and the overex-pression of ubiquitin B had no effect in the cell circle. Western blot results indicated the expression of Smad 4 protein was enhanced in 48hours, and another protein Mcl-1 was attenuated in 48 hours. Conclusion： Ubiquitin B promotes the apoptotic of Hela cells. The possible mechanism is that：ubiquitin B enhanced the expression of Smad 4 and attenuated the expression of Mcl-1, then promoted the apoptotic of HeLa cells.

关 键 词：UBIQUITIN B 泛素 凋亡 宫颈肿瘤细胞 HELA 

分 类 号：R737.31[医药卫生—肿瘤]