顺铂诱导人食管癌Eca-109细胞凋亡的观察  

Observation of cisplatin induced apoptosis of human esophageal carcinoma Eca 109 cells

作　　者：邓丽英[1,2] 张云汉[1,2] 高冬玲[1,2] 许平[1,2] 李欣荣[1,2] 

机构地区：[1]山西长治医学院病理学教研室 [2]河南医科大学第一属医附院病理科

出　　处：《河南医科大学学报》1997年第3期56-59,共4页Journal of Henan Medical University

基　　金：国家"八五"科技攻关项目;河南省重大科技攻关项目

摘　　要：在体外采用光镜、电镜及ＤＮＡ断片检测方法对顺铂作用于人食管癌Ｅｃａ１０９细胞后的形态学及生化改变进行了观察。药物作用后细胞皱缩变小，呈圆形，细胞核固缩、破裂或消失，胞浆红染，有的细胞似出芽状，可见到凋亡小体及凋亡小体被吞噬的现象。顺铂低浓度时凋亡现象不明显，５ｍｇ／Ｌ以上时作用明显。药物作用２４ｈ时经ＤＮＡ琼脂糖凝胶电泳，可见到呈梯状的条带。结果提示：顺铂可引起Ｅｃａ１０９细胞凋亡，ＤＮＡ降解是细胞凋亡过程中的重要变化之一。Morphological and biochemical changes of human esophageal carcinoma Eca 109 cells treated with cisplatin (DDP) were observel in vitro with light and electron microscopes and DNA degradation assay.After treated with DDP,Eca 109 cells displayed retraction,budding,karyopyknosis,karyorrhexis or disappearance of nucleus and dark stained plasm.The formation of apoptotic bodies and phenomena of phagocytosis were apparent.Apoptotic cells at the low concentration of DDP (1 mg/L) were scarce and they were apparent treated with DDP of 5 mg/L or above was increased.DNA ladder was observed at the 24th hour.The results suggest that DNA degradation may be an important change in the process of apoptosis.

关 键 词：顺铂 癌细胞 细胞凋亡 形态学 食管肿瘤 

分 类 号：R735.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]