前列腺癌单克隆抗体与平阳霉素偶联物导向化疗的体外作用  

Effect of antibody-targeted chemotherapy with pingyangmycin on prostate cancer cells in vitro

作　　者：陈维真[1] 张勇[1] 梁昌盛[2] 谢瑶[3] 温新桥[4] 高新[4] 

机构地区：[1]中山大学附属第三医院核医学科,广东广州510630 [2]中山大学基础医学院实验教学中心,广东广州510080 [3]中山大学基础医学院人体解剖学教研室,广东广州510080 [4]中山大学附属第三医院泌尿外科,广东广州510630

出　　处：《南方医科大学学报》2008年第3期406-408,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2003B30103)~~

摘　　要：目的探讨导向化疗对前列腺癌细胞系LNCaP的体外作用。方法以葡聚糖为中介体制备抗前列腺癌单克隆抗体7E11C5.3与平阳霉素(PYM)的偶联物,用间接ELISA法测定其免疫活性,2,3,5-氯化三苯基四氮唑法测定其抑菌活性,四唑盐法测定其体外细胞毒作用。结果偶联物中7E11C5.3和PYM的摩尔比为1:54,偶联后单抗的免疫活性降低了10%￣20%,偶联物中PYM的抑菌活性为游离PYM的25%,偶联物和游离PYM对体外培养的LNCaP细胞50%抑制浓度分别为(9.41±1.98)μg/ml和(29.92±7.88)μg/ml。结论7E11C5.3-PYM偶联物的体外抗前列腺癌作用强于游离PYM。Objective To investigate the effect of antibody-targeted chemotherapy against human prostate cancer LNCaP cells in vitro. Methods The monoclonal antibody 7E11C5.3 against human prostate cancer was conjugated to pingyangrnycin （PYM）, mediated by dextran T-40, and the immunoreactivity of 7E11C5.3 was determined by indirect enzyme-linked irnmunosorbent assay. The bacteriostatic activity of the conjugate was determined using TTC assay, and its cytotoxicity against LNCaP cells was determined by MTT assay. Results The 7El IC5.3：PYM molar ratio was 1：54 in the conjugate, and the immunoreactivity of 7E11C5.3 was decreased by approximately 10% to 20% after conjugation. The bacteriostatic activity of conjugated PYM was 25% of that of free PYM. The 50% inhibitory doses （IC50） of7E11C5.3-PYM conjugate and free PYM against the in vitro cultured LNCaP cells were 9.41±1.98 p,g/ml and 29.92±7.88 μg/ml, respectively. Conclusion 7El IC5.3-PYM conjugate displays stronger cytotoxicity against anti-prostate cancer effects than rice PYM.

关 键 词：前列腺肿瘤 单克隆抗体 平阳霉素 抗体导向化疗 

分 类 号：R737.25[医药卫生—肿瘤]