一种新的检测黄曲霉毒素B1的酶生物传感器的制作(英文)  

The Assembly of a Novel Enzyme Biosensor for Aflatoxin B_1 Detection

作　　者：刘大岭[1] 沈奕[1] 张静[1] 姚冬生[1] 

机构地区：[1]暨南大学生命科学技术学院微生物生物技术研究所,广州510632

出　　处：《中国生物工程杂志》2008年第3期44-52,共9页China Biotechnology

基　　金：国家"863"计划(2002AA213001);国家自然科学基金(30170808);广东省自然科学基金(010418)资助项目~~

摘　　要：报道了一种新的检测黄曲霉毒素B1的生物传感器,该传感器以开管的多壁纳米碳管固定化黄曲霉毒素氧化还原酶制作传感电极检测黄曲霉毒素B1,其线性范围达到0.16μM-3.2μM,当把特异性的黄曲霉毒素B1抗体与黄曲霉毒素氧化还原酶通过多壁纳米碳管共固定化制作修饰电极,传感器的检测限提高到16nM,灵敏度提高了10倍。用这种方法制作黄曲霉毒素酶生物传感器,使黄曲霉毒素酶生物传感器向实用化迈进了一步。A novel biosensor for aflatoxin B1 detecting has been reported. The biosensor electrode for AFB1 detecting was assembled by immobilized aflatoxin-oxidoreductase using open-ended multi-walled carbon nanotubes as matrix. Its linear range was between 0. 161μM and 3.21μM. And if the specific anti-aflatoxin B1 antibody and aflatoxin oxidoreductase were both immobilized on the electrode with Multi-Walled carbon nanotubes, the detection limit of the modified electrode could be 16 nM with a 10 times improved sensitivity. The aflatoxin enzyme biosensor assembled this way strode one step forward its practical application.

关 键 词：黄曲霉毒素B1 酶电极 多壁碳纳米管 

分 类 号：Q819[生物学—生物工程]