ACE基因、CYP11B2基因多态性联合分析与原发性高血压合并缺血性脑血管病的相关性研究  

Study on the association between ACE,CYP11B2 genes polymorphism and Essential Hypertension complicated with lschemic Cerebrovascular Disease

作　　者：吴小盈[1] 李德育[1] 陈慧[1] 骆杰伟[1] 林慧中[1] 陈燕[1] 伍严安[1] 

机构地区：[1]福建医科大学附属省立临床学院,350001

出　　处：《中国分子心脏病学杂志》2008年第1期34-38,共5页Molecular Cardiology of China

基　　金：福建省青年人才科技创新基会2003J051;福建省自然科学基金重点项目C012003

摘　　要：目的探讨血管紧张素转换酶(ACE)基因I/D多态性和醛固酮合酶(CYP11B2)基因-344 T/C多态性联合分析与原发性高血压(EH)及合并缺血性脑血管病(ICVD)的关系。方法运用聚合酶链反应(PCR)、限制性片段长度多态性(RFLP)等技术检测正常对照(NE)组305例、单纯高血压(SEH)组324例和高血压合并缺血性脑血管病(ICVD)组297例的ACE、CYP11B2基因型,结合临床资料统计分析。结果ACE基因DD型及D等位基因频率在ICVD组显著高于NE组和SEH组(P<0.0125),在SEH组与NE组间无显著差异;CYP11B2三种基因型在各组分布差异无显著性;ACE基因DD型+CYP11B2基因TC/CC型分布在三组间有显著差异(P<0.05),DD-TC/CC频率在ICVD组显著高于NE组和SEH组(P<0.0125),在SEH组与NE组间差异无显著性;Logistic回归分析提示,年龄、体重指数(BMI)、血清总胆固醇(CHOL)、血糖(GLU)、基因联合DD-TC/CC是EH人群发生ICVD的独立危险因素,而ACE基因DD型未进入回归方程。结论ACE基因I/D多态性和CYP11B2基因-344T/C多态性与高血压无明显关系,但在高血压人群中,年龄、BMI、CHOL、GLU、ACE基因DD型+CYP11132基因TC/CC型可能是其发生ICVD的独立危险因素,ACE基因与CYP11B2基因可能存在协同作用。Objective To investigate the association between gene polymorphism of angiotension converting enzyme（ACE） and aldosterone synthase（CYP11B2） in essential hypertensives（EH） and patients complicated with ischemic cerebrovascular disease （ICVD） in china. Methods ACE LID polymorphism and CYP11B2 -344T/C polymorphism were detected in 297 ICVD patients, 324 simplex essential hypertensives （SEH） and 305 controls, by using Polymerase Chain Reaction（PCR） and Restriction Fragment Length Polymorphism（RFLP）. Result ACE-DD genotype and D allele frequencies in ICVD were significantly higher than those in SEH and controls, but no significant difference between SEH and controls; CYP11B2 genotype showed no significant difference between all three groups; ACE gene DD ＋ CYP11B2 gene TC/CC combined genotype frequencies in ICVD were significantly higher than those in SEH and controls, but no significant difference between SEH and controls; Logistic regression showed that Age, Body Mass Index （BMI） , serum cholesterol （ CHOL）, plama glucose （ GLU）, and ACE-DD ＋ CYP11B2-TC/CC combined genotype but not the ACE-DD genotype were independent risk factors related to ICVD in EH patients. Conclusion Age, BMI, CHOL, GLU, and ACE gene DD ＋ CYP11B2 gene TC/CC combined genotype may be independent risk factors related to ICVD in EH patients; there may be co-effective action between ACE gene and CYP11B2 gene.

关 键 词：原发性高血压 缺血性脑血管病 ACE基因 CYP11B2基因 

分 类 号：R544.1[医药卫生—心血管疾病] R743.3[医药卫生—内科学]