活性污泥分离混合菌的光合产氢特性分析  

CHARACTERISTIC ANALYSIS ON PHOTOTROPHIC HYDROGEN PRODUCTION BY MIXED CULTURES

作　　者：张立宏[1] 周俊虎[1] 陈明[1] 程军[1] 刘建忠[1] 岑可法[1] 

机构地区：[1]浙江大学能源清洁利用国家重点实验室,杭州310027

出　　处：《太阳能学报》2008年第2期145-151,共7页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(50406022);国家高技术研究发展计划863项目(2006AA05Z122);全国优秀博士学位论文作者专项资金(200437);霍英东高等院校青年教师优选助项目(104021);浙江省科技攻关计划项目(2006C33049)

摘　　要：对从沼气池活性污泥分离出以多种光合细菌为主的混合菌种与纯菌种沼泽红假单胞菌Rh.Palustris Z02进行了光合产氢的实验研究,并利用修正的Gompertz方程进行产氢动力学分析。结果表明,混合菌种比纯菌种的光合细菌表现出更高的产氢能力和更好的稳定性。尤其在对蔗糖和可溶性淀粉的利用上,混合菌种对这两种碳源的利用比较充分,产氢率分别为3.47mol H_2/mol蔗糖和6.68mmol H_2/g可溶性淀粉,氢气含量分别为83.30%和76.06%,气相中没有甲烷气产生。而纯菌种Rh.Palustris Z02产氢率分别只有0.49mol H_2/mol蔗糖和1.95mmol H_2/ g可溶性淀粉。混合菌种的产氢条件较Rh.Palustris Z02更为宽松,以乙酸钠做碳源为例,混合菌种在温度20～40℃,pH=5～8时产氢较好,甚至在pH=5.0和9.0时,仍有微量氢气产生。而纯菌种Rh.Palustris Z02仅在25～35℃产氢较佳,在20℃仅有微量产氢(0.23mol H_2/mol乙酸钠),产氢pH范围6～8,光强产氢范围同混合菌种相似,但是产氢率低于混合菌种。Phototrophic hydrogen production was carried out in batch experiment using mixed cultures extracted from active sludge of methane tank and pure culture Rh. Palustris ZO2. Effect of various parameters, initial pH, temperature, inoculums concentration, light intensity etc, was examined with respect to maximum hydrogen yield. Dynamics of phototropic hydrogen production was investigated using the modified Gompertz equation. The experimental results shown that hydrogen yield of mixed cultures was higher than that of pure culture Rh. Palustris ZO2, especially using sugar and soluble starch as substrate. Mixed cultures utilized these two carbon sources more sufficiently, hydrogen yield was 3.47mol H2/mol sugar and 6.68mmol H2/g soluble starch, and hydrogen content was 83.3O% and 76.O6% respectively. There was no methane gas was detected in the biogas. Hydrogen yield of pure culture Rh. Palustris ZO2 was O.49 mol H2/mol sugar and 1.95mmol H2/g soluble starch. The conditions of hydrogen production using mixed cultures are not strict as using pure cultures, e. g. temperature and pH etc.

关 键 词：产氢 混合菌种 沼泽红假单胞菌 

分 类 号：TQ116[化学工程—无机化工]