丝裂霉素C诱发人精子染色体畸变的研究  

Chromosomal Aberrations Induced in Human Spermatozoaby Mytomicin

作　　者：黄天华[1,2] 刘鸿禧[1,2] 蔡敏[1,2] 黄建民 

机构地区：[1]汕头大学医学院 [2]重庆市计划生育科学研究所

出　　处：《Acta Genetica Sinica》1997年第4期291-295,共5页

基　　金：国家自然科学基金

摘　　要：人精子经最终浓度分别为７．５，１５．３０μｇ／ｍｌ丝裂霉素Ｃ（ＭＭＣ）处理１ｈ后与去透明带地鼠卵受精，制备第１次卵裂中期相进行核型分析，以探讨ＭＭＣ对人精子的诱变效应。结果显示明显的量效关系。染色单体型畸变是诱发畸变的主要类型，但仍观察到一定数量染色体型畸变，表明ＭＭＣ作用于人精子与通常拟紫外线致断剂作用于体细胞诱变的结果并不完全一样。３６．８９％的诱发畸变为重接型。uman spermatozoa were exposed to Mitomycin C (MMC) at the concentrations of7.5,15, 30μg/ml for 1 hour respectivelybefore interspecific in vitro fertilization withzona-free hamster ova. The first cleavage metaphases was analyzed to investigateMMC-induced effects on human spermatozoa. The results showed a distinct dose-responserelationship in the induction of chromosomal aberrations. The chromatid aberrations werethe predominant types in MMC-induced changes. Some chromosome-type aberrations werealso observed. This suggested that the induced effects of MMC on human spermatozoawere some different from that of ultraviolet ligh-like clastogens on somatic cells. 36.89%of MMC-induced aberrations were rejoining-type. This indicated that the DNA repairsystems present in golden hamster oocytes were effective in repairing MMC-induced DNAlesions in human spermatozoa.

关 键 词：丝裂霉素C 人 精子 诱发畸变 染色体畸变 

分 类 号：Q319.31[生物学—遗传学] R321.1[医药卫生—人体解剖和组织胚胎学]