微胶囊固定化过氧化氢酶的制取及对H_2O_2的分解作用  

Decomposition of H 2O 2 by Microencapsulated Catalase

作　　者：刘持标[1,2] 黄葵[1,2] 佘益民[1,2] 单永奎 王玉洁[1,2] 

机构地区：[1]中国科学院长春应用化学研究所 [2]东北师范大学化学系

出　　处：《生物化学杂志》1997年第4期478-482,共5页

摘　　要：以乙基纤维素为膜材，用溶液干燥法制取了微胶囊固定化过氧化氢酶，探讨了制取过程中明胶的加入对微胶囊产率及固定化过氧化氢酶活性的影响，同时论述了存放时间、温度以及环境ｐＨ值对微胶囊固定化过氧化氢酶稳定性的影响．深入研究了微胶囊固定化过氧化氢酶对Ｈ２Ｏ２的分解作用。Catalase was microencapsulated by drying in liquid method with ethyl cellulose as coating material.Microencapsulation of catalase was achieved successfully by the use of gelatin in the presence of the enzyme,which was established to be microencapsulaed in the ethyl cellulose membrane.The fixed catalase showed higher thermal stability than the original enzyme.Furthermore,the stability and activity of the fixed catalase were improved by the use of gelatin.Meanwhile,the catalysis of microencapsulated catalase in H 2O 2 decomposition is studied.Interesting results are found and explained successfully.

关 键 词：微胶囊 过氧化氢酶 过氧化氢 分解 

分 类 号：Q554.603[生物学—生物化学]