红细胞分化因子诱导HL-60细胞排核过程中酪氨酸蛋白质磷酸化的改变  

The Alternation of Tyrosine Protein Phosphorylation of HL-60 during its Denucleation Induced by EDDF

作　　者：郭双立[1] 白龙川[1] 颜卉君[1] 费仁仁 吴国利[1] 

机构地区：[1]北京师范大学生物系生化及分子生物学实验室,北京100875

出　　处：《生物化学杂志》1997年第2期210-215,共6页

基　　金：国家自然科学基金!39370155

摘　　要：红细胞分化因子是从兔子网织红细胞中提出的一个蛋白因子，它可以使多种癌细胞株的生长受到抑制[1]．以早幼粒白血病细胞（HL-60）为材料，研究其被EDDF诱导过程中细胞内PTK，PTPP及它们底物磷酸化水平的变化．实验发现：部分纯化的EDDF对HL-60细胞生长有明显的抑制作用，经NBT还原和Giemsa染色，可见HL-60细胞被诱导出现排核．同时，细胞的PTK，PTPP酶活性有明显的变化，PTPP和PTK的底物蛋白在胞浆中酪氨酸蛋白磷酸化水平亦出现改变（但颗粒部分变化不明显）．Erythroid differentiation factor (EDDF) is a protein factor purified from rabbit reticulocyte. It can inhibit the growth of many kinds of cancer cell line. HL-60 was used as a target cell to studythe alternation of PTPP, PTK activities and the change of the level of their substrate phosphorylation, when it was induced by EDDF. The data demonstrated: partial purified EDDF inhibit the growth of HL-60 apparently. NBT reduction and Giemsa staining showed that EDDF could induce HL-60 denucleation, at the same time the activities of PTPP and PTK change apparently. While the HL-60 was induced to denuclear by EDDF, its tyrosine phosphorylation level decrease in soluble fractiob of the cell, but there was no alternation in the panical fraction.

关 键 词：红细胞分化因子 酪氨酸蛋白 磷酸化 白血病 

分 类 号：R733.702[医药卫生—肿瘤]