内源性生物素在结肠组织中的表达及其对免疫组化的影响  

Expression of endogenous biotin in colonic tissues and its influence on the results of immunohistochemistry

作　　者：高春[1] 王爱英[1] 韩亚京[1] 

机构地区：[1]北京大学第三医院消化科,北京市100083

出　　处：《世界华人消化杂志》2008年第5期551-555,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30571810~~

摘　　要：目的:探讨内源性生物素在结肠组织中的表达及其对免疫组化结果的影响.方法:14例结肠黏膜组织标本按不同的免疫学方法进行实验和分析.结果:8例出现内源性生物素阳性表达(57.14%),主要位于表面上皮细胞胞质,尤其近基底侧.15%蛋清封闭液可以封闭结肠组织中的内源性生物素.传统SP法、SP法+15%蛋清液、PV-6002两步法和改进后的两步法Bax阳性表达数分别为12例(85.71%)、10例(71.43%)、4例(28.57%)和8例(57.14%),在这四种方法中Bax中等强度以上表达数分别为10例、6例、0例和5例.结论:内源性生物素在结肠组织中主要表达在表面上皮细胞胞质,尤其是近基底侧;免疫组化时可首选生物素标记的检测系统.AIM： To investigate the normal expression of endogenous biotin （EB） in colonic tissues and its influence on the results of immunohistochemistry （IHC）. METHODS： Fourteen specimens were tested and analyzed with different immunology methods. RESULTS： EB was expressed on the fundic endoplasm of surface epithelial cells in 8 of the 14 colonic tissue samples, and 15% egg white blocking fluid could eliminate EB in colonic tissues from human beings. Traditional SP, SP ＋ 15% egg white blocking fluid, two-step PV-6002 and improved two-step PV-6002 showed that Bax was positively expressed in 12 （85.71%）, 10 （71.43%）, 4 （28.57%） and 8 （57.14%） colonic tissue samples, respectively, whereas moderately and strongly expressed in 10, 6, 0, and 5 colonic tissue samples, respectively. CONCLUSION： Endogenous biotin is expressed mostly in the fundic endoplasm of surface epithelial cells in colonic tissue samples from human beings. The biotinylated detection system is the first choice of method during IHC.

关 键 词：免疫组织化学 内源性生物素 结肠 石蜡包埋组织 蛋清封闭液 

分 类 号：R574[医药卫生—消化系统]