塞来昔布对胶质瘤U251细胞株放射敏感性影响的实验研究  

Effects of celecoxib on the radiosensitivity of U251 glioma cell line

作　　者：王代旭[1] 黄元奎[1] 牛洪泉[2] 金保山[1] 张华平[1] 潘险峰[1] 

机构地区：[1]华中科技大学同济医学院附属荆州医院神经外科,湖北省荆州市434020 [2]华中科技大学同济医学院附属同济医院神经外科,武汉市430030

出　　处：《实用医学杂志》2008年第5期716-719,共4页The Journal of Practical Medicine

基　　金：荆州市科技局基金资助项目(编号:20071PE1-8)

摘　　要：目的:探讨环氧化酶-2抑制剂塞来昔布(celecoxib)对胶质瘤放射治疗的增敏作用及可能的作用机制。方法:20只裸鼠随机分为4组,每只接种胶质瘤U251细胞4×106个/mL于右后肢皮下。A组用灌肠法给予塞来昔布10mg/(kg.d),并采用6MVX射线照射,5Gy/次,d12、14、16、18,B组仅口服塞来昔布,C组单纯给予放疗,D组未做治疗。测量移植瘤重量,流式细胞术检测各组肿瘤细胞的凋亡,电镜观察各组肿瘤细胞的超微结构,免疫组织化学检测肿瘤共济失调-毛细血管扩张症突变蛋白(ATM)和表皮生长因子受体(EGFR)表达水平。结果:A、B、C、D组肿瘤的重量分别为(54.3±5.95)、(137.2±14.54)、(124.8±19.45)、(193.2±30.42)mg。A组细胞凋亡增多,超微结构可见有凋亡特征的细胞。免疫组织化学检测可见A组肿瘤组织中ATM和EGFR蛋白的表达明显减弱。结论:塞来昔布能够通过降低ATM和EGFR蛋白的表达,增强胶质瘤的放疗敏感性,对胶质瘤的治疗具有重要意义。Objective To investigate the effects of celecoxib on the radiosensitivity of U251 gliomas in vivo. Methods Twenty athymic mice bearing U251 gliomas were randomly divided into four groups, including normal controtreated with radiotherapy （IR） plus celecoxib （A group）, celecoxib alone （B group）, IR alone （C group）, normal saline （D Group）. 4 weeks later after treatment, mice were killed to weigh the tumors. Cell apoptosis was evaluated by flow cytometry and electron microscope. The expressions of ataxia-telangiectasia mutated （ATM） and epidermal growth factor receptor（EGFR） in U251 gliomas were detected by immunohistochmical （S-P） method. Results The tumor weight of A, B,C and D group were （54.3 ± 5.95）, （137.2 ± 14.54）, （124.8 ± 19.45）, （193.2 ± 30.42） mg respectively. Apoptosis was most significant in A group and the expressions of ATM and EGFR were significantly lower than that in D group. Conclusion Celecoxib can promote radiotherapentic sensitivity of gliomas by promoting the expression of ATM and EGFR of gliomas line.

关 键 词：神经胶质瘤 塞来昔布 放射疗法 

分 类 号：R739.41[医药卫生—肿瘤]