自酸蚀牙本质粘接剂对人牙髓成纤维细胞的毒性作用  

Effect of the self-etching adhesives system on human pulp fibroblast

作　　者：张明[1] 冯岩[2] 黄晓晶[1] 雷丽珊[1] 郑碧琼[1] 卢友光[2] 

机构地区：[1]福建医科大学附属口腔医院牙体牙髓科,福建福州350002 [2]福建医科大学附属口腔医院预防科,福建福州350002

出　　处：《华西口腔医学杂志》2008年第1期94-97,共4页West China Journal of Stomatology

基　　金：福建省自然科学基金资助项目(Z0516035);福建省卫生厅青年科研课题资助项目(2007-1-36)

摘　　要：目的比较和评价自酸蚀粘接剂XenoⅢ(XO)和Adper Promp(tAP)以及全酸蚀粘接剂Single bond2(SB)三者的细胞毒性大小。方法将3种牙本质粘接剂XO、AP和SB涂布于直径为5.0mm、厚度为0.5mm的牙本质圆片的两面,置于DMEM培养液中获得材料的浸提液,然后将培养液稀释成100.0%、50.0%、25.0%和12.5%四种体积分数。选用组织块法体外原代培养人牙髓成纤维细胞,并将不同体积分数的材料浸提液与第5代人牙髓成纤维细胞共同培养,通过MTT法评价材料24、72、120h的细胞毒性。结果牙本质粘接系统XO、AP和SB在体外对人牙髓成纤维细胞均有一定程度的细胞毒性,两种自酸蚀粘接剂XO和AP的细胞毒性明显低于全酸蚀粘接剂SB,其差异有统计学意义(P<0.05)。结论自酸蚀牙本质粘接剂XO和AP的细胞毒性小于全酸蚀牙本质粘接剂SB。Objective To compare and evaluate the biocompatibility of three kinds of dentin bonding agents Xeno Ⅲ （XO）, Adper Prompt CAP） , Single bond2CSB） through cell culture in vitro. Methods Three kinds of dentin bonding agents（XO, AP, SB） were applied on the surface of the dental slices which were 5.0 mm in diameter and 0.5 nun in depth. By immersing the slices into the DMEM culture medium, the＇ maceration extracts were obtained. Normal dental pulps of teenagers were collected and human pulp fibroblast was cultured using tissue explant method. The fifth generation pulp cells were exposed to culture medium containing different concentrations of maceration extracts （100.0%, 50.0%, 25.0%, 12.5%） for 24, 72, 120 h. At last, MTT method was used to evaluate the cytotoxicity of the dentin bonding agents on human pulp fibroblast. Results The results showed that all three kinds of dentin bonding systems had cytotoxicity to human pulp fibroblast in different degree in vitro. The cytotoxicity of XO and AP was less than SB. The difference was statistically significant（P〈0.05）. Conclusion The results of cell culture in vitro indicated that total-etching adhesives system has more irritation to pulp than serf-etching adhesives system.

关 键 词：自酸蚀粘接系统 牙髓成纤维细胞 细胞毒性 

分 类 号：R783[医药卫生—口腔医学]