咬合紊乱与去除咬合紊乱对大鼠髁突软骨中骨形成蛋白-2表达的影响  

Effect of induced occlusal disorders and removed occlusal disorders on the expression of bone morphogenetic protein-2 of condylar cartilage in rats

作　　者：李晓峰[1] 王美青[1] 储岚岚[1] 于世宾[1] 

机构地区：[1]第四军医大学口腔医学院解剖生理学教研室

出　　处：《华西口腔医学杂志》2008年第1期105-108,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30471909)

摘　　要：目的探讨咬合紊乱与去除咬合紊乱大鼠髁突软骨内骨形成蛋白-2(BMP-2)的变化。方法幼年和成年雌性大鼠各9只,等分为咬合紊乱组、去除咬合紊乱组和对照组。咬合紊乱组在建立咬合紊乱8周后处死,去除咬合紊乱组在建立咬合紊乱6周时拔除造成紊乱的双侧第一磨牙,2周后处死。对照组不作任何处理,同环境饲养、同期处死。测量各组髁突组织切片上软骨前、中、后部的厚度,SABC法检测软骨前、中、后部BMP-2的表达。结果成年咬合紊乱组髁突软骨中部变薄,后部增厚;去除咬合紊乱后后部恢复正常,中部仍薄于对照组(P<0.05)。幼年髁突软骨的前、中、后部厚度三组间未见显著性差异(P>0.05)。幼年髁突软骨前、中、后部咬合紊乱组BMP-2表达高于去除咬合紊乱组和对照组(P<0.05),成年髁突软骨中部咬合紊乱组和去除咬合紊乱组均高于对照组(P<0.05),后部咬合紊乱组高于和去除咬合紊乱组,后者高于对照组(P<0.05),前部无差异。结论咬合紊乱可导致幼年和成年大鼠髁突软骨BMP-2高表达,成年大鼠髁突软骨对咬合紊乱的适应能力较幼年大鼠差,中部尤为明显。Objective This article was to study the effect of induced occlusal disorders and removed occlusal disorders on the expression of bone morphogenetic protein-2 （BMP-2） of condylar cartilage. Methods Young and adult female Sprague-Dawley rats were divided respectively into induced occlusal disorders group, removed occlusal disorders group and control group, 3 rats every group. For induced occlusal disorders rats, the elastic rubbers were inserted between the first and second molar in the left upper side and right lower side to form the disordered oc- clusion. They were killed under anaesthesia 8 weeks after the treatment. For removed occlusal disorders rats, the first molars that caused disordered occlusion were extracted 6 weeks after forming disordered occlusion. 2 weeks later, they were killed under anaesthesia. For normal rats, they were killed at the same time with experimental rats. Hibateral temporomandibular joints of each rat were removed and stained with HE and monoclone antibody of BMP-2. The thickness of condylar cartilage was measured. The expression of BMP-2 in condylar cartilage was detected by half-quantity immunohistochemical analysis. Results For adult induced occlusal disorders group, the thickness of cartilage in intermediate part of condyle decreased. However, it increased in the posterior part. After removing occlusal disorders, the thickness of posterior condylar cartilage returned to normal level. But it was still thinner than control group in the intermediate part. The expression of BMP-2 in anterior, intermediate, posterior part of condylar cartilage of young induced occlusal disorders group was higher than that of young removed occlusal disorders group and control group. No difference of the expression of BMP-2 was found in young removed occlusal disorders group and control group. For the expression of BMP-2 in intermediate part of condylar cartilage, both adult induced and removed occlusal disorders groups were higher than adult control group. For the posterior part of adult condyle c

关 键 词：下颌骨髁突 关节软骨 骨形成蛋白-2 咬合 

分 类 号：R78[医药卫生—口腔医学]