pLTKcSN/VPC及GCV系统的旁观者效应观察  

Study of bystander effect of the pLTKcSN/VPC and GCV system in vitro

作　　者：张晓鹏[1,2] 胡加飞[1,2] 蔡如珏[1,2] 王伟民[1,2] 袁国梁[1,2] 王驹[1,2] 许秀兰[1,2] 卢亦成[1,2] 张光霁[1,2] 朱诚[1,2] 顾健人[1,2] 

机构地区：[1]第二军医大学长征医院神经外科 [2]上海市肿瘤研究所

出　　处：《第二军医大学学报》1997年第4期325-327,共3页Academic Journal of Second Military Medical University

摘　　要：目的：探讨单纯疱疹病毒Ⅰ型胸苷激酶基因（ＴＫ）逆转录病毒载体生产细胞（ｐＬＴＫｃＳＮ／ＶＰＣ）和更昔洛韦（ＧＣＶ）系统杀伤恶性胶质瘤细胞过程中的旁观者效应。方法：采用大鼠胶质瘤细胞Ｃ６、人恶性胶质瘤Ｕ８７ＭＧ和它们的转染ＴＫ阳性细胞Ｃ６ＴＫ，Ｕ８７ＴＫ，将Ｃ６与Ｃ６ＴＫ，Ｕ８７ＭＧ和Ｕ８７ＴＫ按比例（ＴＫ阳性细胞占细胞总体的０％～１００％，１０％梯度）分别混合培养于９６孔板中，每种混合比例设６孔。培养２４ｈ后，６孔中３孔加入浓度为０．５μｇ／ｍｌＧＣＶ，另外３孔作为空白对照。继续培养７２ｈ，直接计数各孔活细胞数并以对照组计数结果为本底计算ＧＣＶ对各混合比例的抑制率。结果：当ＴＫ阳性细胞占１０％时，Ｃ６和Ｕ８７ＭＧ组的抑制率达到或超过３０％，当ＴＫ阳性细胞占总体的５０％以上时，ＧＣＶ对各混合比例的抑制率接近１００％。结论：ｐＬＴＫｃＳＮ／ＶＰＣ和ＧＣＶ系统杀伤恶性胶质瘤细胞过程中存在明显的旁观者效应。Objective: To evaluate the bystander effect of the vector producer cell of herpes simplex virus Ⅰthymidine kinase gene (pLTKcSN/VPC) and ganciclovir (GCV)system in the killing of malignant glioma cells. Methods: C6 rat glioma cells and C6TK (C6 cells transducted with TK gene ) cells, human malignant glioma cell U87MG and U87TK cells were mixed and cultured in 96 well plates under the condition of 37℃, 5% CO 2. The mixing ratios of TK positive cells accounted for 0%, 10%, 20%,30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%, each mixing ratio had 6 wells . After 24 h of co culture, 0.5μg/ml GCV was added into half of the wells, the other half served as controls. The culture was continued for another 72 h. Direct count of the number of cells alive was carried out in each well, and the supression rate of every mixing status was obtained. Results: When TK positive cells reached 10%, the supression rate of both C6 and U87MG strain run above 30%; When the TK positive ratio reached 50% or more, the tumor killing proportion was almost 100%. Conclusion: Bystander effect of the pLTKcSN/VPC and GCV system could drastically improve the antitumoral efficiency.

关 键 词：恶性 胶质瘤 基因治疗 逆转录病毒 TK GCV 

分 类 号：R730.264[医药卫生—肿瘤] R730.59[医药卫生—临床医学]