三株氧化硫硫杆菌的分离与鉴定(英文)  

Isolation and Characterization of three strains of Acidithiobacillus thiooxidans and its bioleaching of chalcopyrite

作　　者：符波[1] 周洪波[1] 张倩[1] 邱冠周[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《微生物学杂志》2008年第1期12-19,共8页Journal of Microbiology

基　　金：National Nature Science Foundation of Chnia(No50321402);China National Basic Research Program(No2004CB619204)

摘　　要：从煤堆废水中分离得到3株嗜温嗜酸硫氧化细菌。这3株菌株为革兰氏阴性、菌体大小0.4～0.7μm×1～2μm、短杆状运动细菌,其最适生长温度为30℃和最适生长pH2.0～2.5。它们能够利用元素硫,硫代硫酸钠和连四硫酸钾为能源进行自养生长,不能利用有机物质以及硫酸亚铁、黄铁矿和黄铜矿等无机物质作为能源生长。细菌的形态、生理生化特性研究以及基于16SrRNA序列同源性构建的系统发育树结果表明,这3株细菌初步鉴定为氧化硫硫杆菌。氧化硫硫杆菌能够通过产酸有效促进黄铜矿的浸出速率和浸出率。Three strains of mesophilic and acidophilic sulfur-oxidizing bacteria were isolated from coal heap drainage. The bacteria are motile, Gram-negative, rod-shaped, and measures 0.4 to 0.7 by 1 to 2μm. They grow optimally at 30 ℃ and initial pH 2.0 -2.5. The strains grow autotrophically by using elemental sulfur, sodium thiosulfate and potassium tetrathionate as energy sources. The strains can not use organic matter and inorganic minerals including ferrous sulfate, pyrite and chalcopyrite as energy sources. The morphological, biochemical and physiological characterization and analysis based on 16S rRNA gene sequence indicated that the strains are most closely related to AcidithiobaciUus thiooxidans （ 〉 99% similarity in gene sequence）. The presence of sulfur-oxidizing bacteria positively increased the dissolution rate and the percentage recovery of copper from chalcopyrite by producing acid.

关 键 词：ACIDITHIOBACILLUS thiooxidans 分离 16S RRNA 生物浸出 黄铜矿 

分 类 号：Q939.11[生物学—微生物学]