一株产黄纤维单胞菌的选育及产酶特性研究  

Breeding of Cellulomonas flavigena Strain and Its Enzyme-Production Characteristics

作　　者：谢菊兰[1] 肖兵南[1] 周望平[1] 

机构地区：[1]湖南省畜牧兽医研究所,湖南长沙410131

出　　处：《微生物学杂志》2008年第1期41-44,共4页Journal of Microbiology

基　　金：湖南省农业综合开发办资助项目

摘　　要：从土壤中筛选分离出的产纤维素酶菌株S26(经中国科学院微生物所鉴定为产黄纤维单胞菌Cellu-lomonas flavigena),测定酶活为25.86U/mL,以此为出发菌株,经UV反复诱变处理,多代选育,筛选出1株纤维素酶高产突变株UY-4,酶活力达87.92U/mL,是出发菌株的3.4倍,而且遗传性能稳定。对UY-4产酶影响因素的研究表明,产酶最适条件为稻草与麦麸3:2、接种量10%(体积与质量比),(NH4)2SO40.5%、起始pH7.4、35℃、培养72～84h。A cellulase-producing strain S26 was screened from soil （identified as Cellulomonas flavigena by Institute of Microbiology, Chinese Academy of Sciences ） with enzymatic activity tested at 25.86 U/mL. And this strain was taken as starting strain and repeatedly treated with UV to mutate and bred for several generations, a cellulase-high- producing strain UY-4 was obtained with enzymatic activity at 87.92 U/mL, that was 3.4 times that of the starting strain, furthermore, it was genetically stable. Study on the influence factors of enzyme production of UY-4 indicated that the most suitable conditions for the cellulase production were straw ： wheat bran = 3 ： 2, 10% of inoculation （volume/weight） , 0.5 % of （ NH4 ） 2 SO4, 35 ℃ , initial pH at 7.4, culture for 72 - 84 hours.

关 键 词：产黄纤维单胞菌 诱变 纤维素酶 培养条件 

分 类 号：Q55[生物学—生物化学]