敌百虫对小鼠精子的致畸效应及维生素C的拮抗作用  

Teratogenic Effect of Dipterex on Sperm and Antagonistic Effect of Vitamin C in Mices

作　　者：周好乐[1] 郑明霞[1] 于国辉[1] 张三润[1] 杨茜[1] 李杰[1] 

机构地区：[1]内蒙古医学院遗传学教研室生理学教研室,内蒙古呼和浩特010059

出　　处：《内蒙古医学杂志》2008年第2期152-154,共3页Inner Mongolia Medical Journal

基　　金：内蒙古自治区高等学校科学研究项目(NJ06120)

摘　　要：目的:初步探讨敌百虫(dipterex,Dip)对小鼠精子的致畸效应及维生素C的拮抗作用。方法:昆明种小鼠40只,随机分为5组,即对照组、Dip组、维生素C(Vitamin C,VitC)干预组Ⅰ、VitC干预组Ⅱ、VitC干预组Ⅲ,每组8只。对照组和Dip组小鼠每天分别饮用自来水和含Dip的水溶液(5 mg Dip/ml);VitC干预组Ⅰ、VitC干预组Ⅱ和VitC干预组Ⅲ小鼠每天的饮用水中除含有Dip(5 mg Dip/ml)外,同时给予补充不同剂量的VitC进行干预,50 d后观察其精子畸形率的的变化。结果:Dip组小鼠的精子畸形率显著高于对照组(P<0.01);VitC干预组Ⅰ、VitC干预组Ⅱ和VitC干预组Ⅲ的精子畸形率均显著低于Dip组(P<0.01)。结论:Dip的毒性作用可引起雄性小鼠生殖细胞的遗传损伤,导致生殖毒性效应;适量的VitC具有拮抗敌百虫致小鼠精子畸形的作用,即抗诱变作用。Objective：To discuss on the teratogenic effect of dipterex（Dip） in mices and antagonistic effect of vitamin C. Methods： 40 Kunming mices were randomly divided into five groups： control group （n = 8） ,.Dip group（n= 8）, VitC group Ⅰ （n= 8）, VitC group Ⅱ （n = 8）, VitC group Ⅲ （n = 8） .Control group and Dip group were administrated with water and Dip solution, VitC group Ⅰ , Ⅱ and Ⅲ with various doses of vitamin C and Dip. The changes of abberation rate of sperm was observed after 50 days of administrauon. Results： rate of sperm in Dip group was obviously higher（ P 〈 0.01 ） than controls, that in VitC groups was lower than in that in Dip group（ P 〈 0.01）. Conclusion：The toxic effect of Dip induces in genetic damage of reproductive cells and reproductive toxic effect in male mices. Proper VitC can inhibit the effect of Dip on sperum aberration.

关 键 词：敌百虫 维生素C 小鼠 微核 

分 类 号：R394.33[医药卫生—医学遗传学]