欧李组培快速繁殖体系的建立  

Establishment of Rapid Propagation System of Cerasus humilis by Tissue Culture

作　　者：陈书明[1] 姜英淑[2] 王秋玉[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040 [2]北京市林业局种苗站,北京100027

出　　处：《林业科技》2008年第2期59-62,共4页Forestry Science & Technology

基　　金：欧李优良新品种选育及综合利用配套技术开发(2006-38)

摘　　要：以欧李4个品种的茎尖为外植体,1/2MS和MS为基本培养基,初步建立了欧李试管苗的组培快速繁殖体系,其最佳的初代培养基是MS+0.1 mg/L NAA+1.0mg/L 6-BA和MS+0.4mg/L NAA+1.0mg/L 6-BA,最佳的增殖培养基是MS+0.2mg/L NAA+0.8mg/L6-BA和MS+0.2mg/L NAA+0.6mg/L 6-BA+0.05 mg/LPP333,并分别确定了最好的生根培养基。Take 4 species of young stem of Cerasus humilis as explant, and 1/2MS, MS as culture medium. Rapid propagation system of Cerasus humilis by tissue culture was set up. The results showed that the optimal medium for primary culture was MS ＋ 0. 1 mg/L NAA ＋ 1.0mg/L 6 - BA and MS ＋ 0.4 mg/L NAA ＋ 1.0 mg/L 6 - BA. The optimal medium for proliferation were MS ＋ 0.2mg/L NAA0. 8mg/L6 - BA and MS ＋ 0. 2mg/LNAA ＋ 0. 6mg/L6 - BA ＋ 0. 05mg/LPP333. And the best cullture medium has been defined respectively.

关 键 词：欧李 茎尖 组织培养 

分 类 号：S662.5[农业科学—果树学] S603[农业科学—园艺学]