高效液相色谱法测定三棱超临界萃取物中芦丁的含量  

Determination of Rutin in SFE Extract of Sparganium stoloniferum by RP-HPLC

作　　者：李可意[1] 刘红梅[1] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023

出　　处：《时珍国医国药》2008年第3期684-685,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立三棱超临界CO2萃取物中芦丁的鉴别和含量测定方法。方法在聚酰胺膜上点样，以80％乙醇作为展开剂鉴别芦丁，采用SymmetryC18色谱柱，以乙腈-四氢呋喃-0．2％枸橼酸（14：1：85）为流动相，柱温25℃，在流速为1．0ml·min^-1，360nm的检测波长下，测定芦丁含量。结果在三棱的超临界萃取物中含有芦丁成分，建立的液相色谱方法在芦丁浓度为0．00340～0．02432mg·ml^-1的范围内线性关系良好，样品在8h内稳定，平均加样回收率为98．56％，以峰面积作精密度实验，RSD为1．38％。结论三棱的超临界萃取物中含有芦丁，建立的高效液相色谱方法线性关系良好．精密度和回收率高，适合于三棱中芦丁含量的测定。Objective To explore whether there is rutin in SFE extract of Sparganium stoloniferum by TLC and establish a method to determine its content by RP - HPLC. Methods The determination was carried out over a Symmetry C18 column at 25℃. Methods tetrahydrofuran, 0.2% citric acid （14： 1： 85） was used as mobile phase at a velocity of 1 ml · min^-1 and the detection wavelength was set at 360nm. Results Rutin was found in SFE extract of Sparganium Stoloniferum. The calibration curve was linear in the range of 0.003 40 - 0. 02432 mg · ml^-1 for rutin, The sample was stable within 8 hours and the recovery rate of rutin was 98.56 %. And in precision test, RSD = 1.38%. Conclusion There is rutin in SFE extract of Sparganium stoloniferum. The established RP - HPLC method has perfect linear relationship, high precision and recovery rate and it is suitable to determine the content of rutin in SFE extract of Sparganium Stoloniferum.

关 键 词：反相高效液相色谱 薄层色谱鉴别 芦丁 超临界萃取 

分 类 号：R282.5[医药卫生—中药学] R284[医药卫生—中医学]