甲型肝炎减毒活疫苗H2减毒株P2区基因序列测定与特征分析  

Characteristic Analysis on P_2 Gene of Hepatitis A Virus H_2 Live Attenuated Vaccine Strain

作　　者：刘建生[1] 侯宗柳 马绍辉[1] 孟明耀 张云昆[1] 李琦涵[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,云南昆明650118 [2]昆明市延安医院,云南昆明650051

出　　处：《中国疫苗和免疫》2008年第1期7-10,共4页Chinese Journal of Vaccines and Immunization

基　　金：国家自然科学基金项目(30471595);云南省自然科学基金项目(2005C0061M)

摘　　要：目的测定甲型肝炎(甲肝)减毒活疫苗H2减毒株P2区基因序列,了解其减毒和细胞适应的分子机制。方法通过逆转录-聚合酶链反应,特异扩增甲肝减毒活疫苗H2株成品疫苗及其亲本毒种P2区VP1/2A、2B、2C基因序列,经测序、比对,分析检测毒力基因VP1/2A、2C的变异情况。结果甲肝减毒活疫苗H2成品疫苗及其亲本毒种毒力基因VP1/2A、2C区毒力相关基因簇均为减毒型特征。结论甲肝减毒活疫苗H2减毒株毒力基因VP1/2A、2C均为减毒型特征,且遗传稳定性良好。Objective To characterize P2 gene of hepatitis A virus H2 strain which has been applied for live attenuated vaccine production in China. Methods By RT-PCR, specific amplification products of P2 gene from H2 final product and its parent seed were obtained,then sequenced and blasted to analyze its virulence gene VP1/2A and 2C. Results Any nucleotide position clustered as virulence gene among VP1/2A and 2C of H2 final product and its parent seed showed attenuated genotype. Conclusion Hepatitis A virus H2 vaccine strain characterizes a genetic stable and attenuated virulence gene VP1/2A and 2C.

关 键 词：甲型肝炎病毒 H2株 毒力基因 

分 类 号：R373.22[医药卫生—病原生物学]