高效硫酸盐还原菌的分离鉴定及其特性研究  

Isolation and Identification of High Efficient Sulfate-reducing Bacteria and Its Characteristics

作　　者：马保国[1] 胡振琪[1] 张明亮[1] 陈胜华[1] 王萍[1] 黄晓燕[1] 

机构地区：[1]中国矿业大学(北京)土地复垦与生态重建研究所

出　　处：《农业环境科学学报》2008年第2期608-611,共4页Journal of Agro-Environment Science

基　　金：国家高技术研究发展(863)计划项目(2006AA06Z355);教育部新世纪优秀人才计划项目(NCET-04-0484)

摘　　要：从煤矸石山淋溶水长期污染的黄土中分离获得一株硫酸盐还原菌。研究了菌株的生理生态特性,探讨了温度、pH和氧气对菌株的生长及转化硫酸盐效能的影响。结果表明,该菌株是革兰氏阳性菌,可以含有醇羟基的有机碳为碳源(电子供体),硫酸盐等为硫源(电子受体);在有氧和无氧条件下均能生长,菌株为兼性厌氧菌;生长的最适宜pH为7.16;最适生长温度为35℃;硫酸盐转化率最高达94.3%。同时测定了菌株的生长曲线。Sulfate-reducing bacteria （SRB） is isolated from the loess polluted by acid mine drainage in coal mining aerrea in western China. The physiological characteristics of SRB＇ s physiology characteristics and the effect of environmental factors such as temperature, pH and O2 on sulfate reduction are studied. The results show that this strain of SRB is Gram-positive, and can grow with hydroxy organic carbon as electron donors and SO4^2-as electron acceptor under both anaerobic and aerobic conditions. The sulfate is reduced in this process and the rates of sulfate reduction reachs 94.3% .The optimum conditions for SRB growth are found to be at pH 7.16, 35 ℃. The growth curve of SRB is also identified in this paper.

关 键 词：硫酸盐还原菌 菌株分离 生理特性 硫酸盐还原 

分 类 号：X172[环境科学与工程—环境科学]