茶云纹叶枯病病原菌侵入与叶位关系研究  

Studies on the relationship between the infection of Colletotrichum camelliae and different leaf positions

作　　者：高旭晖[1] 郑高云[1] 梁丽云[1] 杨丽丽[1] 杨云秋[1] 

机构地区：[1]安徽农业大学,农业部茶叶生物化学与生物技术重点实验室,合肥230036

出　　处：《植物保护》2008年第2期76-79,共4页Plant Protection

基　　金：安徽省教育厅自然科学重点科研计划项目(2005KJ394ZD)

摘　　要：采用茶园调查和室内接种相结合的方法,研究了茶云纹叶枯病病菌(Colletotrichum camelliae)侵入与不同叶位的关系。结果表明:自然状态下,病害症状主要出现在成、老叶上。2006年嫩叶组、成叶组和老叶组3处理的病叶率分别为0.89%、18.89%、10.67%,2007年分别为2.00%、19.56%、13.78%,其中嫩叶、成叶上的病害都是当年病菌侵染所致。茶枝离体接种时,症状出现在嫩叶和成叶上,而且以前者为多,2006年3处理的病叶率分别为15.28%、9.73%和0%,2007年分别为12.50%、8.73%和2.78%。结果显示:分生孢子的侵染叶位以嫩叶为主,在各类叶片上均有较高的萌发率,但只有部分能够形成附着胞,在嫩叶和成叶上形成率较高,老叶上几乎不能形成。不同叶位的解剖结构如角质层厚薄、栅栏组织层次多少、海绵组织细胞排列松紧等都与病菌侵染有一定的相关性。In vitro inoculations were carried out in the laboratory and field to investigate the relationships between the infection of Colletotrichum camelliae and different leaf positions. Three different treatments were included in the experiments, i. e. tender-leaf group, adult-leaf group and old-leaf group. The results of field investigations showed that the disease incidences in the three treatments were 0.89 %, 18.89% and 10. 67% in 2006, and 2.0%, 19.56% and 13. 78% in 2007, respectively. The symptoms mainly appeared on adult and old leaves. In vitro culture and inoculations in the laboratory showed that the disease incidences in the three treatments were 15.28%, 9.73% and 0% in 2006, and 12. 5%, 8.73% and 2.78% in 2007, respectively. The symptoms appeared on tender and adult leaves. The conidia primarily infected tender and adult leaves, some of which could form appressoria and infected the leaves, but could not on the old leaves. The tissue structures of the leaves at different positions were related to the severity of the disease. For example, the cuticle, palisade, sponge tissue and so on were closely related to the severity of the disease.

关 键 词：茶云纹叶枯病 分生孢子 附着胞 发病率 病情指数 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]