抗创伤弧菌mAb的制备与鉴定  

Preparation and characterization of monoclonal antibody against Vibrio vulnificus

作　　者：庞晓雯[1] 秦红[2] 师建国[1] 

机构地区：[1]第四军医大学基础部病理学与病理生理学教研室,陕西西安710033 [2]第四军医大学基础部解剖学与组织胚胎学教研室,陕西西安710033

出　　处：《第四军医大学学报》2008年第6期532-534,共3页Journal of the Fourth Military Medical University

摘　　要：目的:制备抗创伤弧菌mAb并用于创伤弧菌感染的快速诊断.方法:用灭活创伤弧菌免疫Balb/c小鼠,采用杂交瘤技术制备抗创伤弧菌的mAb,ELISA法用于mAb的Ig效价,亚类及特异性鉴定.结果:获得了2株可持续分泌抗创伤弧菌mAb的杂交瘤细胞株.结论:获得的高特异性的抗创伤弧菌mAb不仅为创伤弧菌感染的快速诊断奠定了坚实的基础,而且为其进一步的治疗方面的研究提供了理论依据.AIM： To prepare monoclonal antibody （ mAb ） against Vibrio vulnificus and evaluate its roles in rapid diagnosis for Vibrio vulnificus infection. METHODS： Balb/c mice were immunized by inactived Vibrio vulnificus. Anti-Vibrio vulnificus McAbs were prepared by using hybridoma technique. The ELISA was used to identify Ig subgroup, titers of ascites, relative affinity and the specificity of McAbs. RESULTS： Two cell lines of hybridoma were established and they constantly secreted high quality McAbs steadily. CONCLUSION： The McAbs established could be highly specific for Vibrio vulnificus, which not only lays a solid foundation for development of a rapid method for detection of Vib- rio vulnificus infection but also provides the theoretical basis for further investigation of the treatment.

关 键 词：弧菌 创伤 抗体 单克隆 杂交瘤 

分 类 号：R392.11[医药卫生—免疫学]