狂犬病病毒糖蛋白基因的克隆及其二级结构和B细胞抗原表位预测  

Cloning, Prediction of the Secondary Structure and B Cell Epitope for the G Protein of Rabies virus

作　　者：李江涛[1,2] 殷相平[3] 柳纪省[3] 胡永浩[1] 

机构地区：[1]甘肃农业大学动物医学院 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [3]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室

出　　处：《动物医学进展》2008年第3期10-13,共4页Progress In Veterinary Medicine

基　　金：国家高技术研究发展计划863项目(2006AA10A204);甘肃省科技攻关(2GS0S2-A41-006-02)

摘　　要：对狂犬病病毒CVS株糖蛋白基因进行克隆与测序，推导出相应的氨基酸序列。然后采用Gamier-Robson法和Chou-Fasman法预测糖蛋白的二级结构，用Kyte-Doolittle法、Emini法和Jameson-Wolf法分别预测蛋白质的疏水性、表面可能性和抗原指数，并综合分析预测糖蛋白的B细胞抗原表位。结果表明，该蛋白结构中含有少量的α螺旋、较多的β折叠以及较为丰富的转角区域，在序列的121～126、133～137、161～165、191～193、201～204、214～216、221～225、264～267区域或其附近最有可能是B细胞抗原表位的优势区域。The G protein gene of rabies virus CVS strain was cloned and sequencea, from which the amino acid sequence was deduced. Then the secondary structure of G protein was predicted by the methods of Gamier-Robson and Chou-Fasman. Hydrophilicity, surface probability and antigenic index were obtained by the methods of Kyte-Doolittle, Emini and Jameson-Wolf, respectively. Combining the results, the B cell epitopes for G protein were predicted. The results showed that the G protein contained some α helix regions, but had much β sheet regions and turn regions. The predominant epitopes of the B cell were probably in the regions of 121-126,133-137,161-165,191-193, 201-204, 214-216, 221-225,264-267.

关 键 词：狂犬病病毒 糖蛋白 二级结构 B细胞表位 

分 类 号：S852.659.5[农业科学—基础兽医学] Q785[农业科学—兽医学]