假单胞菌AB93066产鼠李糖脂发酵条件的优化  

Optimization on Fermentation Conditions of Rhamnolipids Production by Pseudomonas AB93066

作　　者：马满英[1] 施周[1] 刘有势[2] 吴星杰[1] 

机构地区：[1]湖南大学土木工程学院,湖南长沙410082 [2]湖南工业大学科技学院,湖南株洲412011

出　　处：《精细化工》2008年第3期221-225,230,共6页Fine Chemicals

基　　金：教育部博士点基金(20030537370);科技部国际合作基金(2003DFB00002)~~

摘　　要：通过单因素实验和正交实验对铜绿假单胞杆菌AB93066产鼠李糖脂（RL）的摇瓶发酵培养基配方和产生规律进行了研究。结果表明,最佳培养基配方为：ρ（酵母膏）=0.2 g/L,ρ（豆油）=120 g/L,ρ（NaNO3）=6.5 g/L,ρ（KH2PO4）=1.0 g/L,ρ（Na2HPO4·12H2O）=1.0 g/L,ρ（MgSO4·7H2O）=0.1 g/L,ρ（FeSO4·7H2O）=0.2 g/L,pH=6.0。RL的收获期在发酵后156～168 h最佳。发酵生产RL的扩大实验表明,在最优发酵条件下RL的产量可达56 g/L以上,提取后的RL可将去离子水的表面张力降至29.01 mN/m。用高效液相色谱/质谱联用仪进一步分析了所提取的RL的组成,它分别为二鼠李糖脂（R^1）和单鼠李糖脂（R^2）两种同系物。R^1和R^2的表观临界胶束浓度（CMC）分别为0.03 mmol/L和0.04 mmol/L。The optimized conditions for fermentation medium and the production regulation of rhamnolipids（RL） produced by pseudomonas aeruginosa AB93066 were as follows. The composition of the medium was p （yeast） = 0. 2 g/L,ρ （ bean oil） = 120 g/L,ρ （ NaNO3 ） = 6.5 g/L,ρ （ KH2PO4 ） = 1.0 g/L,ρ（Na2HPO4·12H2O） = 1.0 g/L,ρ（MgSO4·7H2O） =0. 1 g/L and ρ（FeSO4·7H2O） =0.2 g/L,pH = 6. 0. The time range for harvesting RL was between 156 h and 168 h after fermentation. Scale-up production experiment indicated that RL yield was 56 g/L and more under the optimal conditions. Surface tension measurements showed that the extracted RL could decrease the surface tension of deionized water to 29. 01 mN/m. Dirhamnolipid （R^1） and monorhamnolipid （R^2） were separated and identified from the extracted RL using liquid chromatography/mass spectrometry. The apparent critical micelle concentration of R^1 and R^2 were 0.03 mmol/L and 0.04 mmol/L.resnectively.

关 键 词：假单胞菌AB93066 生物表面活性剂 鼠李糖脂 发酵条件 

分 类 号：TQ423[化学工程]