人胃癌组织淋巴管新生的超微结构观察  

Ultrastructural studies on initial lymphatics in human gastric carcinoma

作　　者：刘庆仪[1] 陆云飞[1] 覃舒婷[1] 吴非[1] 万福强[1] 严茂军[1] 

机构地区：[1]广西医科大学第一附属医院胃肠外科,广西南宁530021

出　　处：《中华肿瘤防治杂志》2008年第3期211-213,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究人胃癌组织微淋巴管的超微结构特点,探讨胃癌淋巴转移机制。方法:取人胃癌中心区、周边区及正常区组织块,常规固定,树脂包埋,半薄切片,光镜下定位,检出淋巴管经超薄切片,透射电镜观察。结果:人胃癌中心区未见淋巴管;胃癌周边区较正常区微淋巴管增多分别为(3.20±1.152)个和(2.10±0.968)个,t=3.270,P=0.002,管腔较小分别为(204.83±103.71)μm2和(1521.48±794.66)μm2,t=-5.315,P=0.000,开放连接增多(23/59个和4/66个),χ2=12.895,P=0.000,淋巴管破坏增多(38.80%和4.76%),χ2=15.674,P=0.000;淋巴转移组胃癌周边区比无淋巴转移组胃癌周边区淋巴管多分别为(3.50±1.225)个和(2.50±0.548)个,t=2.523,P=0.021,被破坏的淋巴管多(46.15%和13.33%),χ2=5.281,P=0.022,开放连接/非开方连接数差异无统计学意义,χ2=0.217,P=0.641。结论:人胃癌周边区存在淋巴管新生;胃癌细胞进入淋巴管的主要途径可能是胃癌周边区被破坏的淋巴管。OBJECTIVE： To study the ultrastructural features of initial lymphatics in gastric carcinoma and discuss the metastasis mechanisms of malignant cells by lypmphatics. METHODS： The postoperative samples of patients with gastric cancer were obtained in central, peripheral and normal region of cancer. Common resin embedded specimens were made. Lymphatics were localized by hemithin sections under a light microscope and observed by ultrathin sections under a transmission electron microscope. RESULTS： There were no lymphatics in carcinomatous central region. Compared with the normal region, there were more initial lymphatics in peripheral region of gastric carcinoma （3.20±1. 152 vs 2.10±0. 968,t=3. 270, P=0. 002） ,more destroyed lymphatics （38.80% vs4.76%, 2=15.674, P=0.000）, more inter- endothelial openning junctions （23/59 vs 4/66, 2 = 12. 895, P = 0.000）, and the lumina were smaller （204.83 ± 103.71 μm^2 vs 1 521.48±794.66 μm^2,t=-5.315,P=0.000）. Compared with gastric carcinoma without lymph metastasis, there were more lymphatics in peripheral region of gastric carcinoma with lymph node metastasis （3.50±1. 225 vs 2.50±0. 548,t=2. 523, P=0. 021）, more destroyed lymphatics （46.15% vs 13.33%, 2=5.281,P=0.022）, and the interendothelial opening junction were equal, 2 = 0. 217, P = 0. 641. CONCLUSIONS： These results suggest that lymphangiogenesis exists in peripheral region, not central region of gastric carcinoma. Gastric cancer cells may penetrate the lymphatics and get into lymphocinesia via destroyed parts of endothelium in peripheral region of gastric carcinoma.

关 键 词：胃肿瘤 淋巴转移 超微结构 

分 类 号：R735.2[医药卫生—肿瘤]