吲哚美辛对喉癌Hep-2细胞增殖与iNOS活性的影响  

Effects of indomethacin on proliferation and iNOS activity of laryngeal cancer cell Hep-2

作　　者：张连芝[1] 孙吉凤[2] 王少华[1] 王为方[1] 杨柳[1] 王宁[1] 刘剑凯[1] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学教研室,吉林长春130021 [2]长春医学高等专科学校基础医学部生物化学教研室,吉林长春130031

出　　处：《吉林大学学报（医学版）》2008年第2期251-253,共3页Journal of Jilin University：Medicine Edition

基　　金：国家博士后基金资助课题(2004035566)

摘　　要：目的:探讨吲哚美辛对喉癌细胞增殖与诱导型一氧化氮合酶(iNOS)活性的影响,为喉癌的预防和治疗提供理论依据。方法:Hep-2细胞培养于含吲哚美辛(30、60、125和250μmol.L-1)的培养基中,分别培养24h和48h;用MTT方法检测吲哚美辛组细胞增殖的抑制率,用台盼蓝染色法检测吲哚美辛组细胞活力的抑制作用,并与溶剂对照组进行比较;吲哚美辛(125、250和500μmol.L-1)与脂多糖(LPS,10μmol.L-1)同时处理细胞16h,同时设只加LPS对照组,采用酶法和分光光度法检测细胞培养上清液中诱导型一氧化氮合酶(iNOS)的活性。结果:与对照组比较,不同浓度的吲哚美辛(30、60、125和250μmol.L-1)组Hep-2细胞增殖的抑制率均显著增加(P<0.05)。相同浓度的吲哚美辛,随作用时间延长,Hep-2细胞增殖的抑制率显著降低(P<0.05);与对照组比较,不同浓度的吲哚美辛(30、60、125和250μmol.L-1)随剂量增加,Hep-2细胞活力的抑制程度均显著增加(P<0.05)。相同浓度的吲哚美辛,随作用时间延长,Hep-2细胞活力的抑制程度显著降低(P<0.05);与LPS对照组比较,吲哚美辛在125、250及500μmol.L-1浓度时,LPS诱导的细胞培养上清液中iNOS的活性显著降低(P<0.05)。结论:吲哚美辛在30～250μmol.L-1浓度范围内显著抑制Hep-2细胞的细胞增殖与细胞活力,明显降低LPS诱导的细胞iNOS的活性升高。Objective To investigate the effects of indomethacin on proliferation and inducible nitric oxide synthase （iNOS） activity of laryngeal cancer cell Hep-2 to provid theoretical evidence for precaution and healing of laryngeal cancer. Methods Hep-2 cells were exposed to indomethacin at concentrations of 30, 60, 125, 125 μmol·L^-1 and cultivated for 24 and 48 h; MTT and trypan blue exclusion experiments were used to determine cell proliferation and cell viability respectively, and compared with control group. Hep-2 cells were treated with indomethacin （125, 250, 500μmol·L^-1） and lipopolysacchairde （LPS）, 10μmol·L^-1 ） for 16 h, control group with LPS alone was set up. Enzymic method and spectrophotometric method were used to determine the activity of iNOS in cultured supernatant. Results Compared with control group, as the increase of dosage, the inhibitory rates of proliferation of cancer cell Hep-2 in different concentrations of indomethacin groups increased significantly （P〈0.05） . While the identical concentrations of indomethacin were administered for 24 and 48 h, the inhibitory rates of proliferation of cancer cell Hep-2 decreased significantly （P〈0.05） . Compared with control group, as the increase of dosage, the inhibition of cell viability of cancer cell Hep-2 in different concentrations of indomethacin groups decreased significantly （P〈0.05）; Compared with LPS control group, indomethacin （125,250, and 500μmol·L^-1） decreased the LPS-induced iNOS activity significantly （P〈0.05） in the supernatant. Conclusion Indomethacin, within the concentration range of 30-250 μmol·L^-1 , has strong inhibitory effect on Hep-2 cell proliferation and viability and may decrease LPS-induced cell iNOS activity significantly.

关 键 词：吲哚美辛 消炎药 非甾类 喉肿瘤 一氧化氮合酶 

分 类 号：R739.65[医药卫生—肿瘤]