子宫平滑肌激活蛋白-1及间隙连接蛋白43在妊娠期的表达与分娩发动和早产的关系  

Correlation between the expression of activator protein-1 and connexin 43 in uterine smooth muscle during pregnancy and the onset of labor and preterm delivery

作　　者：娄水平[1] 丁依玲[1] 喻玲[1] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011

出　　处：《中华围产医学杂志》2008年第2期73-77,共5页Chinese Journal of Perinatal Medicine

基　　金：湖南省科技厅科研基金（06SK3041）

摘　　要：目的探讨激活蛋白-1（Activator protein-1，AP-1）、间隙连接蛋白43（connexin43，Cx43）在足月产和早产子宫平滑肌中的表达情况及其相关性。方法应用免疫组化法检测15例足月未临产、15例足月临产、10例早产临产产妇的子宫平滑肌中AP-1的两个亚单位c—Jun、c—Fos蛋白及Cx43的表达情况。结果（1）Cx43免疫组化积分在早产临产组（4．20±0．42）及足月临产组（4．33±0．51）表达水平显著高于足月未临产组（3．15±0．41）（P〈0．01）。（2）c—Jun蛋白在早产临产组、足月临产组、足月未临产组标记指数分别为（52．34±4．18）％、（45．25±5．24）％、（34．14±4．26）％，三组两两比较差异均有统计学意义（P〈0．01）。（3）c-Fos蛋白在早产临产组、足月临产组、足月未临产组标记指数分别为（53．48±4．36）％、（43．32±6．21）％、（31．29±3．34）％，三组两两比较差异均有统计学意义（P〈0．01）。（4）妊娠子宫平滑肌中Cx43表达与c—Jun、c—Fos蛋白表达呈正相关关系，（r=0．65、0．63，P〈0．01）。结论Cx43在分娩发动中发挥着重要的作用。妊娠子宫平滑肌中Cx43的表达水平与AP-1表达有一定的相关性。Objective To study the expression of activator protein-1 （AP-1） and connexin 43 （Cx43） in uterine smooth muscle of term pregnancy and its relationship with preterm delivery. Methods Immuno-histochemistry was applied for 15 uterine smooth muscle samples of term pregnancy without labor （Group Ⅰ ）, 15 of term pregnancy in labor （group Ⅱ ） and 10 of preterm delivery in labor （group 1/I ） to investigate the expression of two subunits of AP-1 （c-Jun and c-Fos） andCx43. Results （1） The expression of Cx43 ingroup Ⅰ and Ⅱ （4.33±0.51 and 4. 20±0. 42） were significantly higher than that in group Ⅲ（3.15±0. 41, P±0. 01）. Lable index of c-Jun protein in group Ⅲ , Ⅱ and Ⅰwas （52.34±4.18）% , （45.25±5.24）% and （34.14±4.26）%, respectively （P±0. 01）, and the lable index of c-Fos protein was （53.48±4.36） %, （43.32＋6.21） % and （31.29 ±3.34） %, respectively（P〈0.01）. Positive correlation was found between the expression of Cx43 and c-Jun, c-Fos in pregnancy uterine smooth muscle （r= 0. 65, 0. 63, P〈0. 01）. Conclusions The Cx43 plays an important role in the onset of labor. The expressions of Cx43 is positively related with the expression of AP-1 in pregnancy uterine smooth muscle.

关 键 词：分娩发动 早产 子宫肌层 转录因子AP-1 连接蛋白43 

分 类 号：R739.86[医药卫生—肿瘤] R285.5[医药卫生—临床医学]