多西紫杉醇抑制宫颈癌细胞低氧诱导因子-1α基因低氧性活化的影响  

Inhibition of the hypoxic activation of hypoxia inducible factor-1α by docetaxel in uterine cervical carcinoma

作　　者：蔡昱[1] 

机构地区：[1]湖南省中医药研究院附属医院妇科,湖南长沙410006

出　　处：《现代医药卫生》2008年第8期1109-1111,共3页Journal of Modern Medicine & Health

摘　　要：目的:探讨多西紫杉醇对宫颈癌细胞低氧诱导因子-1α基因表达的影响。方法:Hela细胞在正常条件下(氧气浓度20%)培养后,对照组在正常条件下继续培养24小时,试验组转入缺氧条件下培养24小时,氧气浓度分别为1%。采用反转录聚合酶链式反应(RT-PCR)法检测HIF-1αmRNA表达变化,蛋白质免疫印迹法测定HIF-1α蛋白水平表达变化,MTT法检测肿瘤细胞抑制率。结果:多西紫杉醇可抑制宫颈癌细胞生长,正常对照组有一定水平的HIF-1αmRNA表达,但几乎检测不到HIF-1α蛋白表达,低氧条件下HIF-1αmRNA表达维持在高水平,但HIF-1α蛋白则明显升高(t=2.471～5.474,P<0.01);加入5×10-6mmol/L和20×10-6 mmol/L的多西紫杉醇后则可明显抑制HIF-1α蛋白和mRNA在低氧下的增高,而对常氧下的HIF-1α基因表达无影响。结论:多西紫杉醇可抑制宫颈癌细胞生长,且可抑制HIF-1α基因的低氧性活化。Objective ：To investigate the effect of docetaxel on the hypoxic activation of hypoxia inducible factor（HIF）-1α in uterine cervical carcinoma.Methods：Hela cells were cultured under normoxic condition.The control group was kept under normoxic condition for 24h ,the experimental groups were kept under hypoxic conditions（1% O2） for 24h with or without docetaxel. Growth rate was measured by MMT assay. The HIF-1 α expression was measured by RT-PCR and immunoblot.Results：Docetaxel inhibited the growth of Hela cells. Under normoxic condition, HIF-1α mRNA expression was detected,but not for HIF-1α protein. Under hypoxic condition, HIF-1 protein level increased drastically ,5×10^-6 mmool/L and 20×10^-6 mmol/L of docetaxel significantly inhibited the increase of HIF-1α gene activation under hypoxic conditions （t=2.471-5.474,P〈0.01）. Conclusion：Docetaxel can inhibit the hypoxic activation of hypoxia inducible factor-1α in uterine cervical carcinoma and its growth.

关 键 词：低氧诱导因 宫颈癌 多西紫杉醇 

分 类 号：R73[医药卫生—肿瘤]