大孔树脂吸附分离长春花中的文多灵、长春质碱和长春碱  

Separation of vindoline,catharanthine and vinblastine from Catharanthus roseus (L.) G.Don with macroporous adsorption resin

作　　者：张琳[1] 杨磊[1] 牛卉颖[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040

出　　处：《化工学报》2008年第3期607-614,共8页CIESC Journal

基　　金：国家"十一五"科技支撑项目(2006BAD18B0401);教育部重点基金项目(104191);东北林业大学青年科研基金项目(07041)

摘　　要：通过对11种国产大孔树脂对文多灵、长春质碱和长春碱的静态吸附容量和解吸率等指标的考察,筛选出AB-8大孔吸附树脂作为分离长春花生物碱的载体,其对文多灵、长春质碱和长春碱吸附量分别为365.8、254.2、24.8mg.ml-1。利用大孔吸附树脂吸附分离长春花3种生物碱的过程为:长春花原料用稀硫酸溶液提取,提取液用氨水调节pH值为8,然后采用AB-8大孔吸附树脂柱吸附,用20%乙醇洗涤除去强极性成分,在30℃下用pH=4的50%乙醇解吸,得单吲哚生物碱文多灵和长春质碱,再用90%乙醇解吸,得双吲哚生物碱长春碱。单吲哚生物碱纯度可达50%以上,长春碱纯度达60%以上。Eleven different macroporous adsorption resins were studied with respect to the static adsorption capacity and desorption ratio of vinblastine, vindoline and catharanthine. The best resin was AB-8 and the adsorption capacities of vindoline, catharanthine and vinblastine were 365.8, 254. 2 and 24.8 mg·ml^-1 , respectively. The optimum parameters of separation process by AB-8 resin were as follows： extraction solvent was sulfate dilution; pH value of the extraction was adjusted to 8 by ammonia; elute solvents were 20% alcohol to remove polar components, 50% alcohol （pH= 4） to obtain the mono-indole alkaloids （vindoline and catharanthine） at 30℃ and 90% alcohol to obtain the dimeric-indole alkaloid （vinblastine）. The results showed that the content of mono-indole alkaloids was above 50% and the purity of vinblastine was above 60%.

关 键 词：长春花 文多灵 长春质碱 长春碱 大孔吸附树脂 分离 

分 类 号：TQ028.3[化学工程]