胸水LUNX mRNA检测对肺癌胸膜转移的诊断价值  

Diagnostic value of LUNX mRNA deection pleural metastasis of lung cancer

作　　者：齐学远[1] 陈余清[1] 

机构地区：[1]蚌埠医学院第一附属医院呼吸内科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2008年第2期141-143,146,共4页Journal of Bengbu Medical College

摘　　要：目的：探讨胸腔积液（胸水）LUNX mRNA检测对肺癌胸膜转移的诊断价值。方法：用荧光定量RT-PCR法检测肺癌组44例、肺外肿瘤13例、肺部良性疾病46例及肺外良性疾病11例胸水标本LUNX mRNA表达;用放射免疫分析法检测胸水中癌胚抗原（CEA）、糖类抗原（CA125）、细胞角蛋白19片段（CYFRA21-1）,并进行胸水细胞学检测。结果：肺癌组、肺部良性疾病组胸水LUNX mRNA阳性率及中位拷贝数分别为88.6%、23.58copies/ml和5.26%、0copies/ml;肺外良、恶性疾病组胸水LUNX mRNA阳性率及拷贝数均为0;LUNX mRNA检测的敏感性（88.6%）高于CEA、CA125及CYFRA21-1（40.9%、59.1%、61.3%）（P〈0.05~P〈0.005）;LUNX mRNA阳性率为88.6%,高于细胞学检查的阳性率（65.9%）（P〈0.005）。结论：LUNX mRNA是诊断肺癌胸膜转移特异性较高的分子标志物,对临床胸水性质的鉴别诊断能提供较好的参考价值。Objective：To explore the value of LUNX gene assay in pleural fluid in diagnosis of pleural metastasis of lung cancer. Methods：The LUNX mRNA of 44 pleural effusion samples of lung cancer, 13 pleural effusion samples of the tumor excluding lung tissue and 57 pleural effusion samples of benign disease were detected by real-time RT-PCR. All the pleural effusion samples of lung cancer, CEA, CA125 ,and CYFRA21-1 were tested simultaneously by radioimmunoassay and detected by exfoliative cytology. Results： The positive rate and meso-copies of LUNX gene in lung cancer were 88.6% and 23.58 copies/ml;the positive rate and meso-copies of LUNX gene in benign disease of lung were 5.26% and 0 copies/ml;the positive rate and copies of LUNX gene in tumor and benign disease excluding lung tissue were 0. The sensitivity of LUNX mRNA （ 88.6% ） was significantly higher than that of CEA, CA125 or CYFRA21-1（40.9% ,59. 1% ,61.3% ） （P 〈 0.05 - P 〈 0. 005 ）. Conclusions： LUNX mRNA is a specific molecular marker for diagnosis of pleura] metastasis of lung cancer. It has reference value in differential diagnosis of pleural effusion.

关 键 词：肺肿瘤 胸腔积液 肿瘤转移 肺特异性X蛋白 逆转录聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤] R561.3[医药卫生—临床医学]