血管生成素及其Tie2受体在血管瘤中表达的实验研究  

An Experimental Study on the Expression of Angiopoietin and Tie2 Receptor in Infantile Hemangioma.

作　　者：魏艇[1] 刘文英[1] 位永娟[1] 唐耘熳[1] 周昉[1] 

机构地区：[1]四川大学华西医院小儿外科,成都610041

出　　处：《中华妇幼临床医学杂志（电子版）》2008年第2期9-13,共5页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家教育部博士点基金资助项目(编号:0040205102022);四川省科技厅资助项目(编号:0040205301055)

摘　　要：目的检测血管生成素-1(angiopoietin-1,Ang-1)、血管生成素-2(angiopoietin-2,Ang-2)及其Tie2受体在人体血管瘤裸小鼠移植模型中的表达。方法以裸小鼠为载体,将人体血管瘤组织块移植于裸小鼠的皮下建立动物模型,任其自然生长。于移植后第56、第120天各切取12个移植成活瘤体作RT-PCR荧光定量检测、Western blot免疫印迹和免疫组织化学检测。结果成功建立人体血管瘤裸小鼠移植模型,进一步证实该模型的真实、可靠性。在增生期和消退期移植瘤中,Ang-1的表达低于正常包皮组织,Ang-2和Tie2的表达明显高于正常包皮组织。在移植瘤内发现血管发生这种原始血管生成方式。结论Ang-1,Ang-2及Tie2受体在人体血管瘤裸小鼠移植模型中呈异常表达,移植瘤内存在血管发生这种原始的血管生成方式,提示婴幼儿血管瘤可能不是一种真性肿瘤,而是一种血管生成异常疾病。Objective To investigate the expression of angiopoietin-1 （Ang-1）, angiopoietin-2 （Ang-2） and Tie2 receptor in human hemangioma xenograft animal model on nude mice. Methods The animal model was established by transplanting human hemangioma blocks into nude mice subcutaneously, 12 grafts were harvested on day 56 and 120 after transplantation respectively for fluorescence RT-PCR, Western blot and immunohistochemistry, Results The human hemangioma animal model was successfully established, In proliferating and involuting grafts, the level of Ang-1 mRNA was lower than that of the normal foreskins, the level of Ang-2 mRNA and Tie2 mRNA was higher than that of the normal foreskins, Vasculogenesis was found in the grafts, Conclusion Ang-1, Ang-2 and Tie2 receptor are abnormally expressed in the human hemangiom xenograft animal model, vasculogenesis exists in the hemangioma grafts which is a primitive form of vessel formation, It is proposed that infantile hemangioma is not a real tumor, but a disorder of vessel formation.

关 键 词：婴幼儿血管瘤 血管生成素 TIE2受体 血管发生 血管新生 

分 类 号：R732.2[医药卫生—肿瘤]