妇科恶性肿瘤细胞系中RASSF1A基因启动子区的甲基化及其表达  

Methylation of promoter in RASSF1A gene and its mRNA expression in gynecological malignancy cell lines.

作　　者：王绍海[1] 张萍德[1] 梁铭霖[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科

出　　处：《华中医学杂志》2007年第6期427-428,433,共3页Central China Medical Journal

摘　　要：目的研究Ras相关结构域家族1A基因(RASSF1A)启动子区域在妇科恶性肿瘤细胞中的甲基化状态及其与RASSF1A基因表达的关系。方法采用RT-PCR及甲基化特异性PCR检测9种妇科恶性肿瘤细胞系中RASSF1A基因mRNA表达水平及其启动子区域的DNA甲基化状态。结果A2780、Anglne、CaOV3、SKOV3、HEC-1B和Ishikawa细胞系中RASSF1A基因启动子区域DNA呈现高甲基化,而相应的mRNA表达缺失或低表达,COC1、Hela和Siha细胞系中RASSF1A启动子区域DNA仅部分甲基化,可检测到这些细胞系RASSF1A基因的表达。结论妇科恶性肿瘤细胞系普遍存在RASSF1A基因启动子区域DNA的高甲基化,这可能在妇科恶性肿瘤的发生发展中起作用。Objective To explore methylation status of Ras-association Domain Family 1A Gene （RASSF1A） in promoter region and its expression at mRNA level in gynecological malignancy cell lines. Methods RT - PCR and Methylation-specific PCR were performed to detect RASSF1A gene expression and DNA methylation status of its promoter region in 9 cell lines as followes： A2780, Anglne, CaOV3, COC1, SKOV3, Hela, HEC-1B, Ishikawa, and Siha. Results RASSF1A gene promoter region showed DNA hypermethylation and low levels or loss of mRNA expression was found in A2780, Anglne, CaOV3, SK- OV3, HEC-1B and Ishikawa cells, whereas partial DNA methylation was identified and its mRNA detected in COC1, Hela and Siha cells. Conclusion DNA mehtylation in RASSF1A gene promoter region is ubiquitous in gynecological malignancy cell lines, which could play some role in pathophysiology of these cancers.

关 键 词：妇科恶性肿瘤细胞系 RASSF1A基因 DNA甲基化 

分 类 号：R737.3[医药卫生—肿瘤]