兔VX-2肝移植肿瘤的制作方法研究  

Study of preparation of rabbit model with VX-2 carcinoma in liver

作　　者：王宝剑[1] 刘林祥[1] 林少华[2] 刘艳丽[3] 

机构地区：[1]泰山医学院医学影像诊断学教研室,山东泰安271016 [2]荣成市人民医院内科,山东荣成264300 [3]山东大学医学院解剖教研室,山东济南250012

出　　处：《泰山医学院学报》2007年第10期776-779,共4页Journal of Taishan Medical College

摘　　要：目的探索应用不同方法制作VX-2兔移植性肝癌模型,评价原位成瘤率。方法新西兰白兔38只,采用VX-2瘤株动物自身接种传代。A组15只采用超声引导瘤组织混悬液注射法;B组13只采用开腹瘤组织混悬液注射法;C组10只采用瘤组织块包埋法,均接种于肝左叶。各组分别于接种后1周、2周、3周、4周、5周行CT平扫、增强扫描及MR I平扫。计算各组的原位成瘤率。结果A、B组各种植成功10只,C组种植成功7只,分别为66.7%、76.9%、70%,无统计学意义。结论三组制作方法都有较高的原位成瘤率,实验周期短,均可用于兔VX-2肝癌模型的制作。Objective： To study the methods of preparing the rabbit model with VX-2 carcinoma in liver used in the experimental radiology and evaluate the original growth rate of each group. Methods： 38 New-Zealand white rabbits were divided into 3 groups. 15 New-Zealand white rabbits were inoculated with suspension with Ultrasound transduce, 13 were inoculated with suspension by laparotomy and 10 were inoculated with VX-2 carcinoma in the liver by laparotomy implantation. All were inoculated in the left lobe of the liver and examined by plain and dual-phase enhanced CT scan and MRI plain scan respectively in 1 week, 2 weeks, 3 weeks, 4 weeks, and 5 weeks later. The original growth rate of VX-2 carci- noma in each group was calculated. Results ： VX-2 carcinoma in liver was found successfully in ten rabbits of both Group A and Group B; for Group C, VX-2 carcinoma in liver was found successfully in seven rabbits . Conclusion： The original growth rate of VX-2 carcinoma is high by the methods in all the groups, and experimental period is short. The methods are effective for the research of liver neoplasm.

关 键 词：兔 肝 VX-2肿瘤 动物模型 体层摄影术 X线计算机 磁共振成像 

分 类 号：R735.7[医药卫生—肿瘤]