乳腺癌转移抑制基因1对乳腺癌细胞运动能力的影响  

Effect of breast cancer metastasis suppressor gene 1 on the migration of breast cancer cells

作　　者：杨银龙[1] 翟羽[1] 邵志敏[1] 

机构地区：[1]复旦大学附属肿瘤医院乳腺外科,复旦大学上海医学院肿瘤学系,上海200032

出　　处：《肿瘤》2008年第3期207-210,共4页Tumor

基　　金：国家重大科学研究计划资助项目(编号:2006CB910501);国家自然科学基金资助项目(编号:30572109);上海市科学技术委员会资助项目(编号:06DZ19504)

摘　　要：目的:研究乳腺癌转移抑制基因1(breast cancer metastasis suppressor gene1,BRMS1)对人乳腺癌细胞运动能力的影响。方法:构建BRMS1真核表达载体,利用Lipofectin2000转染肺高转移潜能乳腺癌细胞MDA-MB-231-HM,荧光实时定量PCR检测目的基因表达,Transwell实验检测细胞运动能力。同时,设计3对针对BRMS1的特异小干扰RNA(small interfering RNA,siRNA),通过荧光实时定量PCR筛选出最有效的siRNA,干扰人乳腺癌细胞MDA-MB-231,观察干扰后细胞运动能力的变化。结果:与转染空载体的细胞相比,稳定转染BRMS1的MDA-MB-231-HM细胞穿过膜的数量减少51.9%。筛选出1对有效siRNA能明显下调BRMS1,用此siRNA干扰MDA-MB-231细胞后,其穿过膜的细胞数量增加17.9%。结论:BRMS1能抑制人乳腺癌细胞的运动,提示其可能通过抑制人乳腺癌细胞运动而抑制肿瘤远处转移。Objective： To study the effect of breast cancer metastasis suppressor gene 1 （BRMS1） on the migration of human breast cancer cells. Methods：An eukaryotic expression vector containing BRMS1 was constructed and was transfected into human breast cancer MDA-MB-231-HM cells by using Lipofectin 2000. Expression of BRMS1 mRNA was detected by real-time PCR. The migration of the cells was assayed by Transwell test. Meanwhile, three pairs of siRNA targeting BRMS1 were designed. The most effective siRNA was screened by real-time PCR and transfected into MDA-MB-231 cells. The migration ability of the transfected cells was detected. Re- suits：Compared with the cells transfected with empty vector, the number of MDA-MB-231-HM cells on the underlayer of transwell de- creased by 51.9% after stably transfected with BRMS1. One pair of siRNA was selected which could notably down-regulated BRMS1 expression at mRNA level. After siRNA transfection, the number of MDA-MB-231 cells on the underlayer of transwell increased by 17.9%. Concluslon：BRMS1 suppresses the migration of breast cancer cells, which indicates that BRMS1 may suppress cancer distant metastasis by inhibiting migration of breast cancer cells.

关 键 词：乳腺肿瘤 肿瘤转移 乳腺癌转移抑制基因1 

分 类 号：R737.9[医药卫生—肿瘤]